Light Absorption Measurement in the Clinical Chemistry Solution Using CCD Line Scan Sensor

Abstract

This research paper describes how to measure the light absorption in clinical chemistrysolution by CCD line scan sensor. The CCD line scan sensor is employed to measure a spectrum(wavelengths ranging from 400 to 800 nanometer) instead of using momochromator system whichmeasured only a wavelength at a time. Using the CCD line scan sensor is more convenient and fasterat a time without moving of any accessories even when changing the wavelength of measurement,and compute measure the narrow bandwidth.

The Halogen lamp is chosen as the light source passed through the clinical chemistry solutionand then collimator to focus the light into a parallel beam which in turn passed through diffractiongrating to produce the spectrum of light. The spectrum of each different wavelength are gathered bya convex lens converging line sensor to measure the light intensity. The calculation of light absorptionis then processed by a microcomputer. The results of experiment can be measured the lightabsorption by using the 3,000 pixels of the line sensor. It is also presented the error of wavelengthonly 0.049% while comparing with the mercury lamp. According to Beer and Lambertûs law, theabsorption value of light depends on the concentration of solution in linear equal ratio.