Screening of Pb-Tolerant Plant Growth-Promoting Rhizobacteria from Roots of Pb-Excluder, Grown on Pb Contaminated Soil

  • Jiraporn Yongpisanphop Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand
  • Sandhya Babel School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani 12120, Thailand
Keywords: Excluder, Lead contaminated soil, Pityrogramma calomelanos, Plant growth-promoting trait, Rhizospheric bacteria

Abstract

Bacteria are microbes that can be used as natural tools for phytoremediation. The present study was conducted to discover a new species of Pb-tolerant siderophore producing and non-phosphate solubilizing rhizobacteria for phytostabilization. In this study, a Pb-phytostabilizer (Pityrogramma calomelanos) rhizobacterium, grown on Pb contaminated soil, was isolated and characterized genotypically and phenotypically. No isolate diversity was found in a harsh environment containing high, total and extractable Pb concentrations up to 45500 and 621 mg/kg, respectively. Only the S3 isolate was found and selected. This rhizobacterial isolate showed remarkable Pb tolerance up to 1850 mg/L. Moreover, it produced siderophores and could not solubilize phosphate. Interestingly, these properties support the immobilization of Pb. This isolate was identified as a strain that is closely related to Arthrobacter humicola by partial 16S rRNA gene analysis. To the best of our knowledge, this is the first study proposing a new candidate, A. humicola, for assisting in Pb phytostabilization. The potential use of this rhizobacterial candidate should be further investigated, using a pot experiment.

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Published
2021-03-16
How to Cite
Yongpisanphop, J., & Babel, S. (2021). Screening of Pb-Tolerant Plant Growth-Promoting Rhizobacteria from Roots of Pb-Excluder, Grown on Pb Contaminated Soil. Science & Technology Asia, 26(1), 96-106. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/225856
Section
Engineering