Effects of NAA BA and Sucrose on Shoot Induction and Rapid Micropropagation by Trimming Shoot of Curcuma longa L.

Abstract

Shoot tips of Curcuma longa L. were used as explants. These explants were cultured on MS medium supplemented with various concentrations of (0, 1, 2, 3 and 4 mg/l) BA and (0, 0.5, and 1 mg/l) NAA. They were significant difference (p≤0.05) in each parameter. Explants which were cultured on MS medium supplemented with 1 mg/l NAA and 2 or 3 mg/l BA gave the highest average number of new shoots ( 2.4, 2.6 shoots, respectively) and number of leaves (5.4 leaves), optimum number of roots (2.6 roots per shoot), and plant height (4.5 cm). MS medium supplemented with only 2 mg/l BA produced the highest average number of shoot (2.6 shoots) and 5.4 leaves per shoot. Explants were trimmed in longitudinal sections (LS) to 2 and 3 sections. At 2 sections gave the highest number of new shoots (4.3 shoots per section). Explants which were cultured on MS medium with 60 gm/l gave the highest average shoots and leaves per bunch, and longest and biggest size of root.

Keyword: Curcuma longa L.; BA; NAA; sucrose; trimming