@article{Trieu Ly_Le_2021, title={Preliminary Phytochemical Analysis and the Anti-Diabetic Effect of Leaf Extracts of Symplocos cochinchinensis (Lour.) Moore ssp. Laurina (Retz.) Nooteb. Against α-Amylase and α-Glucosidase}, volume={26}, url={https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/241491}, abstractNote={<p>One of the ways of controlling postprandial glucose level is to inhibit carbohydratehydrolyzing enzymes like <em>α</em>-amylase and <em>α</em>-glucosidase. This study was undertaken to provide <em>in vitro</em> evidence for the potential inhibitory activity of crude extract and different fractions of <em>Symplocos cochinchinensis</em> leaves on <em>α</em>-amylase and <em>α-</em>glucosidase enzymes. Crude and fractionated extracts were obtained by percolation and liquid-liquid extraction. <em>In vitro</em> antidiabetic activity of all extracts was assessed based on <em>α</em>-amylase and <em>α</em>-glucosidase inhibition. Furthermore, phytochemical screening was done via chemical reactions, and the total polyphenol (TPC) and flavonoid (TFC) contents were measured via colorimetric methods using <em>S. cochinchinensis</em> leaves. Results showed that <em>S. cochinchinensis</em> leaves contain polyphenols, flavonoids, saponins, triterpenoids, alkaloids, anthraglycosides, anthocyanosides, proanthocyanidins, tannins, polyuronics, and reducing agents. The TPC was 517.71 mg gallic acid equivalent/g dry mass while the TFC was 1.12 mg quercetin equivalent/g dry mass. The results indicated that crude extract and its fractions demonstrated inhibitory activities on both <em>α</em>-amylase and <em>α</em>-glucosidase, of which, the crude extract and ethyl acetate fraction had the highest inhibitory potential for both <em>α</em>-amylase and <em>α</em>-glucosidase of all the fractions tested, with IC<sub>50</sub> values of 38.85 and 35.74 μg/mL (crude extract), 30.18 and 30.91 μg/mL (ethyl acetate fraction), respectively. These fractions were especially better than acarbose (45.49 and 53.18 μg/mL). Simultaneously, there was a significant correlation between the TPC of the <em>S. cochinchinensis</em> leaf extracts and their <em>α</em>-amylase and <em>α</em>-glucosidase inhibitory activities. Further <em>in vivo</em> studies should be performed to clarify the regulating effect of <em>S. cochinchinensis</em> leaves on postprandial blood glucose.</p>}, number={3}, journal={Science & Technology Asia}, author={Trieu Ly, Hai and Le, Vu Khanh Trang}, year={2021}, month={Sep.}, pages={174–187} }