TY - JOUR AU - Thongphueak, Dueantem AU - Chansiri, Kosum AU - Sriyapai, Thayat AU - Areekit, Supatra AU - Santiwatanakul, Somchai AU - Wangroongsarb, Piyada PY - 2019/02/08 Y2 - 2024/03/29 TI - Development of the Rapid Test Kit for the Identification of Campylobacter spp. Based on Loop-mediated Isothermal Amplification (LAMP) in Combination with a Lateral Flow Dipstick (LFD) and Gold Nano-DNA Probe (AuNPs) JF - Science & Technology Asia JA - Sci Tech Asia VL - 24 IS - 1 SE - DO - UR - https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/171212 SP - 63-71 AB - <p>The detection of <em>Campylobacter</em> spp. in meat products was developed by using loop-mediated isothermal amplification (LAMP) combined with DNA-based bioassay methods, including a lateral-flow dipstick (LFD) and gold nano-DNA probe (AuNPs) assay. The LAMP primers were designed from the conserved nucleotide regions of <em>Campylobacter</em> spp. The analytical sensitivity of the LAMP-LFD and LAMP-AuNPs analysis was 360 fg/μl. The analytical specificity of LAMP-based assays showed no cross-reactions to <em>Listeria monocytogenes</em>, <em>Salmonella Typhimurium</em>, <em>Escherichia coli</em>, <em>Bacillus cereus</em>, <em>Pseudomonas aeruginosa</em>, <em>Staphylococcus aureus</em>, <em>Enterobacter aerogenes</em>, <em>Serratia marcescens</em>, <em>Vibrio parahaemolyticus</em>, <em>Vibrio cholerae</em>, <em>Klebsiella oxytoca</em> and <em>Citrobacter diversus</em>.The sensitivity, specificity and accuracy of both LAMP-LFD and LAMP-AuNPs for the detection of pre-enrichment cultures from raw chicken meat samples were 100%, 95% and 96.67%, respectively. Since the processing time of LAMP-based assays is 60-90 minutes, it is applicable as a point-of-care screening test for food safety and as a process control of <em>Campylobacter </em>spp<em>. </em>contamination.</p> ER -