TY - JOUR AU - Tongrueng, Surapong AU - Vongpralub, Thevin AU - Srimooltho, Watcharaporn AU - Phasuk, Yupin PY - 2021/03/16 Y2 - 2024/03/29 TI - Transforming Growth Factor Beta1 in Porcine Seminal Plasma on Characteristic of Sperm and Reproductive Efficiency in Sows JF - Science & Technology Asia JA - Sci Tech Asia VL - 26 IS - 1 SE - DO - UR - https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/225956 SP - 216-223 AB - <p>Transforming growth factor-β1(TGF-β1) in porcine seminal plasma (SP) and TGF-β1 concentrations under different storage periods at 16°C were investigated. The effects of adding TGF-β1, to boar semen, on sperm characteristics and reproductive performance in sows were also examined. TGF-β1 in boar SP was measured by enzyme-linked immunosorbent assay (ELISA) technique. Mean concentrations of TGF-β1 in individual ejaculated SP samples demonstrated a wide variation at 8.94-588.24 pg/mL with a decrease in concentration of 46.10±5.52% after 72 hours. The supplementation of TGF-β1 to fresh semen at 0, 1000, and 2000 pg/100 mL was performed in order to evaluate its effect on sperm characteristics. Results showed a significant difference at 24 hours in the following characteristics: viability, progressive, and acrosome integrity, viability, velocity average path (VAP), and velocity curvilinear (VCL) at 72 hours (P&lt;0.05). Acrosome integrity, mitochondrial function, motility, velocity average path (VAP), velocity straight line (VSL), and velocity curvilinear (VCL) were not significantly difference at 24 hours (P&gt;0.05). Mitochondrial function, progressive motility, and VSL were not significantly different at 72 hours (P&gt;0.05). In addition, the supplementation of TGF-β1 at 0, 500, and 1000 pg/100 mL of fresh boar semen for artificial insemination in sows did not result in a significant difference in conception rate, farrowing rate, litter size, or total born alive (P&gt;0.05).</p> ER -