Differential Phosphoproteomics Analysis to Identify Proteins and Pathways of Human Lung Epithelial Cells During Exposure to Burkholderia Pseudomallei
Keywords:
Burkholderia psudomalliei, Bp1026b, A549, Phosphoprotein, Human lung epithelialAbstract
Burkholderia psudomalliei (Bp) is the causative agent of melioidosis, an infectious disease of humans and animals. At present there is no available human vaccine that protects against Bp, and with the current limitations of antibiotic treatment, the development of new preventative and therapeutic interventions is crucial. This study elucidated human epithelial host cell responses immediatly after exposure to Bp. Forty-five phosphoproteins of human lung epithelial cells were activated after exposure to Bp. Seven cellular pathway were found to be associated with 8 of those activated phosphoproteins. The results suggested the response of the host cell including cell proliferation, gene expression, differentiation, mitosis, cell survival, energy production and apoptosis. These results suggested the rapid response of the host cell and the possible importance of phosphoproteins in host responses to pathogen. Moreover, those phosphoproteins can be fulfill basic knowledge to insight host response to control Bp infection.
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