Standardized Karyotype and Idiogram of White Eye Barb (Cyclocheilichthys repasson) (Cypriniformes, Cyprinidae) in Thailand by Conventional and Ag-NOR Staining Techniques
Keywords:
Cyclocheilichthys repasson, Chromosome, Ag-NOR stainingAbstract
The conventional and Ag-NOR banding techniques were performed in the white eye barb (Cyclocheilichthys repasson) from the Chao Phraya River at Ton Pho Subdictrict, Muang Singburi Dictrict, Sing Buri Province in the central of Thailand. The mitotic chromosome preparations were directly obtained from kidney tissues of eight males and eight females. The results showed that the diploid chromosome number of C. repasson was 2n=50, the fundamental number was 96 in both male and female. The karyotype of chromosomes consisted of six large metacentric, eight large submetacentric, two large acrocentric, six medium metacentric, 14 medium submetacentric, 10 medium acrocentric and four medium telocentric chromosomes. There are no irregularly sized chromosomes related to sex. The regions adjacent to the short arm near the telomere of metacentric chromosome pair 1, submetacentric chromosome pairs 9 and 12, and acrocentric chromosome pair 18 showed clearly observable nucleolar organizer regions (NORs). The karyotype formula for C. repasson is as follows:
2n (diploid) 50 = Lm6 + Lsm8 + La2 + Mm6 + Msm14 + Ma10 + Mt4 or 12m + 22sm + 12a + 4t
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