Properties and Antioxidant activities of Hydrogel Containing Sericin from J108 Cultivar

Authors

  • Thanapha Leartchayantee สาขาวิชาเภสัชเคมี คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น
  • Suthasinee Thapphasaraphong ผู้ช่วยศาสตราจารย์ ภาควิชาเภสัชเคมี คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น
  • Nattawadee Kanpipith นักศึกษา หลักสูตรปรัชญาดุษฎีบัณฑิต สาขาวิชาชีวเวชศาสตร์ บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแก่น

Keywords:

Sericin hydrogel, Cross-linking, Swelling

Abstract

In this study was to extract sericin from J108 silkworm and determine MW and protein content. The physicochemical properties of hydrogel were also investigated.  The results were found that sericin extracted from J108 silkworm cultivar showed the same range of MW as sericin from commercial as 25-150 kDa. The protein content of sericin extracted from J108 was 34.11±1.07 µg/mL whereas commercial sericin was 4.53±0.52 µg/mL From FT-IR spectra, both sericin showed some different peak positions which told the different functional groups. To study antioxcidant activities of sericin hydrogel, the hydrogel from sericin extracted from J108 silworm cultivar(HGJ) showed higher antioxidant activities by FRAP ssay and DPPH assay and provided higher crosslink reaction and viscosity than hydrogel from commercial sericin (HGC) but showed lower swelling property than HGC. The results revealed that structure of sericin effected to the formation and properties of hydrogel. Furthermore, the cultivars, extraction mehods and sericin concentration also be considered  for the suitable hydrogel properties for cosmetic products applications.

References

Kumar JP, Mandal BB. Antioxidant potential of mulberry and non-mulberry sericin and its implications in biomedicine. Free Radic Biol Med. 2017; (108): 803-818.

Aramwit P, Angkasirisap W, Srichana T. The effect of silk sericin protein on collagen production and skin wound healing in rats. KKU Res J. 2009; 14(5): 460-470.

Manosroi A, Boonpisuttinant K, Winitchai S, Manosroi W, Manosroi, Manosroi J. Free radical scavenging and tyrosinase inhibition activity of oil and sericin extracted from Thai native silkworm (Bombyx mori). Pharm Biol. 2010; 48(8): 855-860.

Aramwit P, Towiwat P, Srichana T. Anti-inflamatory potential of silk sericin. Nat Pro Comn. 2013; 8(4): 501-504.

Senakoon W, Nuchadomrong S, Sirimungkararat S, Senawong T, Kitikoon P. Antibacterial action of eri (Samia ricini) sericin against esterichia coli and staphylococcus aureus. As J Food Ag-Ind. 2009; special issue: 222-228.

Chirani N, Yahia LH, Gritsch L, Motta FL, Chirani S, Faré S. History and Application of hydrogel. J Bioned Sci. 2015; 2:(13): 1-23

Cho KY, Moon JY, Lee YW, Lee KG, Yeo JH, Kweon HY, et al. Preparation of self-assembled silk sericin nanoparticles. Int J Biol Macromol. 2003; 32(1-2): 36-42

Mohite PB, Adhav SS. A hydrogel: Methods of preparation and application. IJAP. 2017; 6(3): 79-85.

Hennick WE, Nostrum CFV. Novel crosslinking methods to design hydrogel. Adv Drug Deliv Rev. 2002; 54(1): 13-16.

Tanun W, Sangsuwan S. Super Absorbent Polymer: Synthesis, Characterization, and Applications. JSSE. 2014; 16(2): 63-81.

Chuasinuan P, Techasakul S, Nooeiad P. Antibacterial hydrogel of Thyme Essential Oil-incorporated Gelatin/Silk sericin as wound dressing. SWU Sci J. 2017; 33(2): 1-15.

Shukla R, Kashaw SK, Jain AP, Lodhi S. Fabrication of Apigenin loaded gallan gum-chitosan hydrogels (GGCH-HGs) for effective diabetic wound healing. Int J Bio Macromol. 2016;( 91): 1110-1119.

Hyon SH, Cha WI, Ikada Y, Kita M, Ogura Y, Honda Y. Poly (vinyl alcohol) hydrogels as soft contact lens material. J Biomater Sci Polym Ed. 1994; 5(5): 397-406.

Lee TW, Kim CH, Hwang SJ. Hydrogel patches containing Triclosan for acne treatment. Eur J Pharm Biopharm. 2003; 56(3): 407-412.

Parente ME, Andrade OA, Ares G, Russo F, Jiménez-Kairuz á. Bioadhesive hydrogels for cosmetic applications. Int J Cosmet Sci. 2015; 37(5): 511-518.

Aramwit P, Siritientong T, Kanokpanont S, Srichana T. Formulation and characterization of silk sericin-PVA scaffold crosslink with genipin, Int J Biol Mocromol. 2010; (47): 668-675.

Ramos-de-la-Pen˜a AM, Renard CMGC, Montan˜ez J, Reyes-Vega MdlL, Contreras-Esquivel JC. A review through recovery, purification and identification of genipin. Phytochem Rev. 2014; 15(1): 37-49.

Winotapun W, Opanasopit P, Ngawhirunpat T. Oneenzyme catalyzed simultaneous plant cell disruption and conversion of released glycoside to aglycone combined with in situ product separation as green one-pot production of genipin from Gardenia fruit. Enzym Microbial Technol. 2013; 53: 92-96.

Wang QS, Xiang Y, Cui YL, Lin KM, Zhang XF. Dietary blue pigment derived from genipin, attenuate inflammatory by inhibiting LPS-indiced iNOS and COX-2 expression via the NF-kB inactivation. PLOS ONE. 2012; 7(3): e34122.

Aramwit P, Damrongsakkul S, Kanokpanont S, Srichana T. Properties and antityrosinase activity of sericin from various extraction methods. Biotechnol App Biochem. 2010; 55(2): 91-98.

Liliam KH, Laura IL, Alessandra C, Erica C, Welida F, Tatiane P, et al. Sericin from Bombyx mori cocoons. Part I: Extraction and physical-biological characterization for biopharmaceutical application. Process Biochem. 2017; (61): 163-177.

Bradford MM. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the principle of protein-dye binding. Anal Chem. 1976; (72): 248-254.

Mi FL, Sung HW, Shyu SS. Synthesis and Characterization of a Novel Chitosan-Based Network Prepared Using Naturally Occuring Crosslinker. J Polym Sci A. 2000; (38): 2804-2814.

Shi Y, Xiong D, Liu Y, Wang N, Zhao X. swelling mechanical and friction properties of PVA/PVP hydrogels after swelling in osmotic pressure solution. Mater Sci Eng C. 2016; (65): 172-180.

Benzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a measure of “Antioxidant power” : The FRAP Assay. Anal Chem. 1996; (239): 70-76.

Brand-William W, Cuvelier ME, Berset C. Use of Free Radical Method to Evaluate Antioxidant Activity. Lebensm-Wiss. u.-Technol. 1995; (28): 25-30.

Yuan Y, Chesnutt BM, Utturkar G, Haggard WO, Yang Y, Ong JL, Bumgardner JD. The effect of cross-linking of chitosan microspheres with genipin on protein release. Carbohydr Polym. 2007; (68): 561-567.

Mu C, Zhang K, Lin W, Li D. Ring-opening polymerization of genipin and its long-range crosslinking effect on collagen hydrogel. J Biomed Mater Res A. 2013; 101(2): 385-393.

Hobanthad T. Extraction of sericin from Thai native silk (Nangsew) yarn using mulberry (cv. Buriram 60) leaves extracts as a greener solvent. SNRU JST. 2018; 10(3): 156-164.

Zhang W, Ren G, Xu H, Zhang J, Liu H, Mu S, Cai X, Wu T. Genipin-cross-linked chitosan hydrogel for the controlled release of tetracycline worth controlled release property, lower cytotoxicity, and long-term bioactivity. J Polym Res. 2016; 23(156): 1-9.

Klein MP, Hackenhaar CR, Lorenzoni ASG, Rodrigues RC, Costa TMH, Ninow JL, Hertz PF. Chitosan crosslinked with genipin s support matrix for application in food process: Support characterization and β-D galactosidase immobilization. Carbohydr polym. 2016; (137): 184-190.

Schiffman JD, Schauer CL. Cross-Linking Chitosan Nanofibers. Biomacromolecule. 2007; (8): 594-601.

Yan LP, Wang YJ, Ren L, Wu G, Caridade SG, Fan JB, Wang LU, Ji PH, Oliviera JM, Oliviera JT, Mano JF, Reis RL. Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering application. J Biomed Mater Res A. 2010; 95(2): 465-475.

Mandal BB, Priya AS, Kundu SC. Novel silk sericin/gelatin 3-D scaffolds and 2-D films: Fabrication and characterization for potential tissue engineering applications. Acta Biomater. 2009; (5): 3007-3020.

Nadzir MM, Mun LS, Juan CP. Characterization of Genipin-Crosslinked Gelatin Hydrogel Loaded with Curcumin. JEAS. 2017; 12(9): 2294-2298.

Prior RL, Wu X, Schaich K. Standardized Methods for the Determination of Antioxidant capacity and Phenolics in Food and Dietary Supplements. J Agric Food Chem. 2005; (53): 4290-4302.

Kumar JP, Mandal BB. Antioxidant potential of mulberry and non-mulberry silk sericin and its implications in biomedicine. Free Radical Bio Med. 2017; (108): 803-818.

Downloads

Published

2020-09-17

Issue

Section

บทความวิจัย