Formalin-killed blood-stage Plasmodium yoelii Ajuvanted with CpG Oligodeoxyneucleotide and Montanide Induces Protective Immune Responses Against Malaria Infection in Mice

Abstract

This study aimed to investigate the immune response of BALB/c mice after immunization with formalin-killed blood stage P. yoelii formulated with Montanide ISA720 plus CpG ODN. The results showed that immunization with 3x107formalin-killed P. yoelii-pRBC formulated with the adjuvants induced P. yoelii-specific antibody responses and protected mice against live P. yoelii challenge infection. The lower numbers of formalin-killed P. yoelii-pRBC with those adjuvants were not able to induce any antibody response and protection against challenge infection either. The determination of P. yoelii-specific antibody isotypes of the immune mice showed that IgG1 antibody titers were highest, followed by IgG2a, IgG2b and IgG3.