Proteomics and Transcriptomics Studies in Nonreplicating Persistence of Mycobacterium tuberculosis Beijing and Non-Beijing Strains

Authors

  • Bhanubong Saiboonjan Department of Microbiology, Faculty of Medicine, Khon Kaen University 40002
  • Sittiruk Roytrakul National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Rama VI Rd., Pathumthani, Thailand
  • Arunnee Sangka Assistant Professor, Department of Clinical Microbiology, Faculty of Associated Medical Science, Khon Kaen University, Thailand
  • Viraphong Lulitanond Professor, Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Kiatichai Faksri Associate Professor, Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
  • Wises Namwat Assistant Professor, Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

Keywords:

Mycobacterium tuberculosis, Multiple stresses, Beijing

Abstract

Latent tuberculosis infection (LTBI) is an infectious disease which the pathogen can behave as non-replicating persistent (NRP) or dormancy cells, and it caused by Mycobacterium tuberculosis (Mtb) strains. Beijing and non-Beijing strains have been reported and suggested to facilitate rise of the re-infection rate in regions with a high incidence of TB disease. To better understanding of molecular mechanisms and explore biomarker of the pathogen via NRP stage, mimicking condition is thought to reflect of Mtb inside the host granulomas (intracellular environment). The experiments have been designed by hypoxia, low pH, iron deprivation and nutrient starvation. Proteins prepared from multiple stress cultures, were then performed in-gel digestion with Trypsin and characterized by Liquid chromatography tandem mass spectrometry and then validated by quantitative real-time PCR. The results showed that strains Beijing found to be up-regulation of cyp123, Rv1496, ppgK and folP1 and non-Beijing found to be up-regulation of mce1R and Rv1501. The unique feature of proteins presents in this study that allowed the investigation and identification of Mtb strains during multiple stresses. Which further benefit for identification of potential biomarkers lead to discovery new drug targets and vaccine development.

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Published

2021-05-19

Issue

Vol. 21 No. 2 (2021)

Section

บทความวิจัย