High Performance Liquid Chromatography (HPLC) Fingerprint Study and Analysis of Aloin and Piperine Contents From Thor-Ra-Nee-San-Ta-Kat (TRK) Formula in Thailand
Keywords:
Aloin, Piperine, Thor-Ra-Nee-San-Ta-Kat FormulaAbstract
Thor-Ra-Nee-San-Ta-Kat (TRK) is an ancient herbal formula used for the treatment of various diseases with the indication from the list of essential herbal medicinal products for the treatment of degenerative diseases. TRK is manufactured and distributed in hospitals and Thai traditional clinics for the treatment of chronic muscle pain and constipation as well as large-scale production in pharmaceutical factories. This research is the high-performance liquid chromatography (HPLC) fingerprint study and analysis of aloin and piperine from an in-house TRK product compared with ten TRK products from hospitals and commercial sources. The results exhibited six distinct HPLC peaks from TRK1-TRK11 at the retention time of 2.567, 3.421, 11.782, 12.985, 14.970, and 49.752. The HPLC peaks at retention times 12.985 and 14.970 were identified as aloin A and aloin B, respectively whereas the peak at 49.752 was pinpointed as piperine. The marker analysis showed that the average aloin content and piperine content in TRK products were 16.524 ± 0.41 and 25.590 ± 037 % dry weight, respectively. The results from the study will be useful for the standardization and quality control together with the increment of confidence, the efficiency, and the safety of TRK products in the future.
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