Human Invariant Natural Killer T (iNKT) cells response to Burkholderia thailandensis in vitro
Keywords:
iNKT cells, B. thailandensis, B. pseudomallei, CD69, Activation, PBMCsAbstract
Burkholderia thailandensis is a non-pathogenic bacterium closely related to Burkholderia pseudomallei, the causative agent of melioidosis. Invariant Natural Killer (iNKT) cells are subpopulation of T lymphocytes that express natural killer receptors and unique T cell receptors that recognized lipid antigen. Upon activation, iNKT cells rapidly produced and secreted a number of cytokines such as IL-4 and IFN-g to modulate the other immune cells. The iNKT cells play a crucial role in several bacterial infections. However, the human iNKT cells response to B. thailandensis infection has never been investigated. Accordingly, this study purposed to investigate iNKT cells response to B. thailandensis in vitro. Heat-killed B. thailandensis (HKBT) was used to stimulate human peripheral blood mononuclear cells, the iNKT cells number and activated levels were evaluated by flow cytometer compared to heat-killed B. pseudomallei (HKBP) and unstimulated controls. Results showed that the percentage of number iNKT cells in total T cells were all the same (0.40%, 0.41% and 0.41%) in with HKBT and HKBP and unstimulated control. We found similar result when the percentage of number iNKT cells was calculated as absolute lymphocytes (0.18% for PBS, 0.15% for HKBT and 0.18% for HKBP). Interestingly, when the percentage of activated cells (%CD69+ iNKT+ cells) was analyzed, HKBT gave higher stimulation (48.7%) than those of HKBP (34.6%) and unstimulated control (0.64%). The components of B. thailandensis that associated with iNKT cells stimulation were found to relate with nucleic acid and proteins. Our finding demonstrated that HKBT could activate human iNKT cells stronger than HKBP. This dissimilar might describe the different in host immune stimulation leading to better bacteria clearance of B. thailandensis hence make it not cause disease.
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