High Performance Liquid Chromatography (HPLC) Analysis of Active Compounds Contents from SAHATHARA Formula in Thailand
Keywords:
High performance liquid chromatography, Piperine, SAHATHARA formulaAbstract
Sahasthara is categorized as Thai traditional medicine containing white pepper as an active ingredient for the treatment of muscle and bone disorders under the National List of Essential Medicines. This research study aimed to analyze piperine as an active ingredient of Sahasthara capsules with High-Performance Liquid Chromatography to compare the amount of the active ingredient contained in Sahasthara capsules available in the market and in-house. The results showed that the amount of piperine contained in the prepared Sahasthara capsule was 1.44±0.00% dry weight. Moreover, according to the analysis of 7 samples of Sahasthara capsules available in the market, SAH06 contained the largest amount of piperine (1.15±0.01% dry weight), and SAH02 contained the least amount of Piperine (0.87±0.01% dry weight). This resulted in a statistical significance difference (p-value ≤ 0.05). The findings of the study can result in the quality control of raw materials to achieve standards and good quality.
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