ฤทธิ์ทางชีวภาพและสารออกฤทธิ์ทางชีวภาพของพืชสมุนไพรไทยกระชายขาว (Boesenbergia rotunda (L.) Mansf):

Sunthorn Chooluck; Busarakum Pomthong; Natapat Sapim; Wichuda Jankangram

Authors

Sunthorn Chooluck อาจารย์ ภาควิชาชีวเคมี คณะวิทยาศาสตร์ มหาวิทยาลัยบูรพา
Busarakum Pomthong อาจารย์ คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏนครราชสีมา
Natapat Sapim นิสิต หลักสูตรปริญญาวิทยาศาสตรบัณฑิต ภาควิชาชีวเคมี คณะวิทยาศาสตร์ มหาวิทยาลัยบูรพา
Wichuda Jankangram ผู้ช่วยศาสตราจารย์ คณะวิทยาศาสตร์และสังคมศาสตร์ มหาวิทยาลัยบูรพา วิทยาเขตสระแก้ว

Keywords:

Boesenbergia rotunda (L.), Biological activity, Antioxidant activity

Abstract

Boesenbergia rotunda (L.) Mansf., more often known as white galingale, is a medicinal plant with bioactive compounds able to inhibit free radicals. Using alternative herbal medications offers good efficacy as well as great safety for health compared to chemically manufactured drug treatments. In this research, antioxidant capacity, total phenolic content, flavonoid content and tyrosinase inhibitory assay of B. rotunda (L.) Mansf. were studied in relation to extraction duration. Ground, dried white galingale obtained from Sa Kaeo Province cooperatives were extracted by ethanolic maceration for 3, 6, 9, 12, and 24 hours respectively. The total phenolic content of all extracts were determined and extraction for 3 hour has the highest value (6.80 ± 0.07 mg GAE/g DW). Extraction for 12 hours yielded the highest total flavonoid content (2.14 ± 0.02 mg QE/g DW). Extraction for 24 hours resulted in the maximum antioxidant activity (80.2 ± 0.7% inhibition) with the ABTS assay while extraction for 3 hours appears to possess the highest antioxidant activity (87.68 ± 0.34% inhibition) for DPPH assay. The most powerful reducing ability of 64.70 ± 15.28 g/ml was found after 24 hours of extraction using a potassium ferricyanide reducing power test. Bradford assay revealed that 24 hour extracts contain highest protein content (12.22 ± 0.32 mg BSA/g DW). The tyrosinase inhibitory activity was also analyzed using dopachrome method. The results suggested that 3 hour extracts possessed the highest inhibitory effect (67.33 ± 0.85% inhibition).

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