Influence of different extraction solvents on antioxidant and antityrosinase activities of Morus alba Linn. leave extract.
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Abstract
The objective of this study was to investigate the impact of different extraction solvents such as hexane, benzene, ethyl acetate, acetone, ethanol, and water on antioxidant and antityrosinase efficiency of mulberry leave extract. The sample was extracted from leaves by maceration method. The obtained extract was filtered and evaporated to dryness by using the rotary evaporator prior to measuring the total phenolic content, flavonoids content, antioxidant activities, antityrosinase activities, and morin content in triplicates. The results indicated that the highest antioxidant activity was found in ethyl acetate (EA) fraction of CM60 with ABTS value of 57.8±7.7 % inhibition and DPPH value of 58.2±0.7 %inhibition that were positively related to its phenolic content (36.9±1.3 mg GAE/mg DW). Interestingly, The highest antityrosinase activity was found in acetone fraction of CM60 which inhibited tyrosinase activity by 3.0±0.1 mg KE/mg DW that was positively related to its flavonoids content (32.71±0.1 mg QE/mg DW) and was related to its highest morin content measured using HPLC (0.71±0.01 mg/mg DW). The present results advocate that the EA fraction of CM60 possessed the highest antioxidant property related to its phenolic content, and the acetone fraction showed the highest antityrosinase activity related to its flavonoids content especially morin which is tyrosinase inhibitor. Both fractions of M. alba were potential candidates for skin protector from oxidative damage and skin-whitening agent development. Further studies are necessary to formulate the compounds responsible for antioxidant and antityrosinase properties and to investigate antityrosinase properties in vivo prior to transferring technology to communities.
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Abarca-Vargas, R., Peña Malacara, C.F. and Petricevich, V.L. 2016. Characterization of chemical compounds with antioxidant and cytotoxic activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) extracts. Antioxidants. 5(4). doi:10.3390/antiox5040045.
Agorku, E.S., Kwaansa-Ansah, E.E., Voegborlo, R.B., Amegbletor, P., and Opoku, F. 2016. Mercury and hydroquinone content of skin toning creams and cosmoetic soaps, and the potential risks to the health of Ghanaian women. SpringerPlus. 5: 319. doi: 10.1186/s40064-016-1967-1. eCollection 2016.
Andallu, B., Shankaran, M., Ullagaddi, R., and Iyer, S. 2014. In vitro free radical scavenging and in vivo antioxidant potential of mulberry (Morus indica L.) leaves. Journal of Herbal Medicine. 4(1): 10-17.
Andarwulan, N., Batari, R., Sandrasari, D.A., Bolling, B., and Wijaya, H. 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chemistry. 121(4): 1231–1235.
Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R., and Koirala, N. 2019. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants (Basel). 8(4): doi:10.3390/plants8040096.
Bae, S.H., and Suh, H.J. 2007. Antioxidant activities of five different mulberry cultivars in Korea. LWT- Food Science and Technology. 40(6): 955-962.
Chang, L-W., Juang, L-J., Wang, B-S., Wang, M-Y., Tai, H-M., Hung, W-J., Chen, Y-J., and Huang, M-H., 2011. Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food and Chemical Technology. 49: 785-790.
Chang, Y.C., Yang, M.Y., Chen, S.C., Wang, C.J. 2016. Mulberry leaf polyphenol extract improves obesity by inducing adipocyte apoptosis and inhibiting preadipocyte differentiation and hepatic lipogenesis. Journal of Functional Foods. 21: 249-262.
Chauhan, S., Verma, S. C., and Kumar, V. 2013. Evaluation of phytochemical and anti-oxidant activity in different mulberry varieties. Asian Journal of Chemistry. 25(14): 8010-8014.
Chon, S.U., Kim, Y.M., Park, Y.J., Heo, B.G., Park, Y.S., and Gorinstein, S. 2009. Antioxidant and antiproliferative effects of methanol extracts from raw and fermented parts of mulberry plant (Morus alba L.). European Food Research and Technology. 230: 231-237.
Cui, H., Lu, T., Wang, M., Zou, X., Zhang, Y., Yang, X., Dong, Y., and Zhou, H. 2019. Flavonoids from Morus alba L. leaves: Optimization of extraction by response surface methodology and comprehensive evaluation of their antioxidant, antimicrobial, and inhibition of α-amylase activities through analytical hierarchy process. Molecules. 24: 2398. doi:10.3390/molecules 24132398.
Hao, J.Y., Wan, Y., Yao, X.H., Zhao, W.G., Hu, R.Z., Chen, C., Li, L., Zhang, D.Y., and Wu, G.H. 2018. Effect of different planting areas on the chemical compositions and hypoglycemic and antioxidant activities of mulberry leaf extracts in Southern China. PLoS One. 13(6): e0198072. doi: 10.1371/journal.pone.0198072.
Hashemi, S., and Khadivi, A. 2020. Morphological and pomological characteristics of white mulberry (Morus alba L.) accessions. Scientia Horticulturae. 259: 108827. https://doi.org/10.1016/j.scienta.2019.108827
Hussain, F., Rana, Z., Shafique, H., Malik, A., and Hussain, Z. 2017. Phytopharmacological potential of different species of Morus alba and their bioactive phytochemicals: A review. Asian Pacific Journal of Tropical Biomedicine. 7(10): 950-956.
Kim, H.B. 2017. Comparison with various mulberry leaves' and fruit's extract in lipid accumulation inhibitory effect at adipocyte model. International Journal of Industrial Entomology. 35: 1-6.
Kim, I., and Lee, J. 2017. Comparison of different extraction solvents and sonication times for characterization of antioxidant activity and polyphenol composition in mulberry (Morus alba L.) Applied Biological Chemistry. 60: 509-517.
Li, H., Zhang, D., Tan, L-H., Yu, B., Zhao, S-P., and Cao, W-G. 2017. Comparison of the antioxidant properties of various solvent extracts from Dipsacus asperoides and identification of phenolic compounds by LC-ESI-QTOF-MS–MS. South African Journal of Botany. 109:1-8.
McGregor, D. 2007. Hydroquinone: An evaluation of the human risks from its carcinogenic and mutagenic properties. Critical Reviews in Toxicology. 33(10): 887-914.
Momtaz, S., Mapunya, B.M., Houghton, P.J., Edgerly, C., Hussein, A., Naidoo, S., and Lall, N. 2008. Tyrosinase inhibition by extracts and constituents of Sideroxylon inerme L. stem bark, used in South Africa for skin lightening. Journal of Ethnopharmacology.119(3):507-12. doi: 10.1016/j.jep.2008.06.006. Epub 2008 Jun 13.
Nasti, T. H. and Timares, L. 2014. Invited Review MC1R, Eumelanin and Pheomelanin: their role in determining the susceptibility to skin cancer. Photochemistry and Photobiology. 91(1): 188-200
Parvez, S., Kang, M., Chung, H.S., Cho, C., Hong, M.C., Shin, M.K., and Bae, H. 2006. Survey and mechanism of skin depigmenting and lightening agents. Phytotherapy Research. 20(11): 921-34.
Ranjan, B., Kumar, R., Verma, N., Mittal, S., Pakrasi, P.L., and Kumar, R.V. 2017. Evaluation of the antidiabetic properties of S-1708 mulberry variety. Pharmacognosy Magazine. 13: 280-288
Reichardt, C. 2003. Solvents and Solvent Effects in Organic Chemistry, 3rd ed., Wiley-VCH Verlag, Weinheim, Germany.
Riche, D.M., Riche, K.D., East, H.E., Barrett, E.K., and May, W.L. 2017.Impact of mulberry leaf extract on type 2 diabetes (Mul-DM): A randomized, placebo-controlled pilot study. Complementary Therapies in Medicine. 32: 105–108.
Ribeiro, A. S., Estanqueiro, M., Oliveira, M. B., and Lobo, J. M. S. 2015. Main Benefits and Applicability of Plant Extracts in Skin Care Products. Cosmetics. 2, 48-65.
Saravanakumar, K., Sarikurkcu, C., and Sarikurkcu, R.T. 2019. A comparative study on the phenolic composition, antioxidant and enzyme inhibition activities of two endemic Onosma species. Industrial Crops and Products. 142: 111878.
Sassa-deepaeng, T., Khamphira, T., Yodthong, W., and Wanapichit, V. 2017. Antibacterial antioxidant composition of Eclipta prostrata. Proceeding of 4th Conference on Research and Creative Innovations and 2nd International symposium on Application of High-voltage, Plasma and Micro/Nano Bubble to Agriculture and Aquaculture. 26-27 Jul 2017. Chiangmai Grandview Hotel and Convention Center, Chiang Mai, Thailand. p.664-671.
Sassa-deepaeng, T., Yodthong, W., and Khamphira, T. 2019. Green synthesized copper nanoparticles and their anti-bacterial properties against bullfrog multidrug resistant gram negative bacteria. Veterinary Integrative Sciences. 17(1): 33-49.
Sheng, Y., Zheng, S., Zhang, C., Zhao, C., He, X., Xu. W., and Huang, K. 2018. Mulberry leaf tea alleviates diabetic nephropathy by inhibiting PKC signaling and modulating intestinal flora. Journal of Functional Foods. 46: 118-127.
Solano, F. 2014. Melanins: Skin Pigments and Much More—Types, Structural Models, Biological Functions, and Formation Routes. New Journal of Science. Article ID 498276, doi.org/10.1155/2014/498276. Epub.
Sun, F., Shen, L., and Ma Z. 2011. Screening for Ligans of human aromatase from mulberry (Mori alba L.) leaf by using high-performance liquid chromatography/tandem mass spectrometry. Food Chemistry. 126: 1337-1343.
Thavamoney, N., Sivanadian, L., Tee, L.H., Khoo, H.E., Prasad, K.N., and Kong, K.W. 2018. Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents. Journal of Food Science and Technology. 55(7): 2523-2532.
Ulbing C. K. S., Muuse, J.M., Miner, B.E. 2019 Melanism protects alpine zooplankton from DNA damage caused by ultraviolet radiation. Proceedings of the Royal Society B: Biological Sciences. 286: 20192075. https://dx.doi.org/10.1098/rspb.2019.2075 Epub.
Ya, W., Chun-Meng, Z., Tao, G., Yi-Lin, Z., and Ping, Z. 2015. Preliminary screening of 44 plant extracts for anti-tyrosinase and antioxidant activities. Pakistan Journal of Pharmaceutical Sciences. 28(5): 1737-1744.
Yadav, A.V., and Nade, V.S. 2008. Anti-dopaminergic effect of the methanolic extract of Morus alba L. leaves. Indian Journal of Pharmacology. 40(5): 221-226.
Yang, J. Ou, X.Q., Zhang, X., Zhou, Z.Y., and Ma, L.Y., 2017. Effect of Different Solvents on the Measurement of phenolics and the antioxidant activity of mulberry (Morus atropurpurea Roxb.) with accelerated solvent extraction. Journal of Food Science. 82(3): 605-612. doi: 10.1111/1750-3841.13638. Epub.
Yun, H.Y., Kim, D.H., Son, S., Ullah, S., Kim, S.J., Kim, Y.J., Yoo, J.W., Jung, Y., Chun, P., and Moon, H.R. 2015. Design, synthesis, and anti-melanogenic effects of (E)-2-benzoyl-3-(substituted phenyl) acrylonitriles. Drug Design, Development and Therapy. 4(9): 4259-4268.
Zhang, H.W.; Chen, X.Y. and Ling, C.Y. 2010. Optimization of alcoholic extraction of flavonoids from mulberry leaves using response surface methodology. Amino Acids and Biotic Resources. 34: 76–79.
Zhang, L., Tao, G., Chen, J., and Zheng, Z-P. 2016. Characterization of a new flavone and tyrosinase inhibition constituents from the twigs of Morus alba L. Molecules. 21, 1130; doi:10.3390/molecules 21091130.
Zou, Y., Liao, S., Shen, W., Liu, F., Tang, C., Chen, C-Y.O., and Sun, Y. 2012. Phenolics and Antioxidant Activity of Mulberry Leaves Depend on Cultivar and Harvest Month in Southern China. International Journal of Molecular Sciences. 13(12): 16544–16553.