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Vanda is one of the orchid genera that are important commercial crops. It was traded in both cut flower and plant form. In 2018, the Vanda export of Thailand had a marketable value of about 0.13% as cut flowers and 8.9% as orchid plants. Orchid cultivation is usually used a large number of fertilizers for growth and development, which lead to high cost and environmental problems. Therefore, this research was aimed to study N2- fixing and IAA produced by endophytic bacteria as biofertilizers under greenhouse conditions. This research was conducted in a completely randomized design (CRD), and Vanda plantlets from tissue culture were inoculated with isolated 3S19 endophytic bacteria compared with deionized water ( as a control treatment). Each treatment had twenty replications. The results showed that there were no significantly different between isolate 3S19 inoculation plants and with sterilized deionized water in plant height and leave number at 2, 3, and 4 months after inoculation. In contrast, root length and the number of roots were significantly different at 4 months after inoculation. The data from this research is beneficial as primary data for biofertilizer application to Vanda orchid.
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