Production of Xylotetraose from Agricultural Residues by Using Xylanolytic Enzyme from Thermophilic Anoxybacillus sp. Strain JT-12

Article Sidebar

FULL TEXT IN THAI

Main Article Content

Jitladda Thitikorn-amorn
King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150
Khin Lay Kyu
King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150
Khanok Ratanakhanokchai
King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150
Yun-Sik Lee
Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, South Korea

Abstract

Among 21 microorganisms isolated from a soil sample of pulp and paper industry, a thermophilicAnoxybacillus sp. strain JT-12 was found to produce xylotetraose as the main product during cultivation onmedium containing birchwood xylan as a sole carbon source. Hydrolysis of agriculture residues such asrice husk, corn hull, corn cob, rice straw and bagasse by the crude xylanolytic enzyme (consist of 3 types ofxylanases, β-xylosidase, arabinofuranosidase and acetylesterase) was investigated at pH 7.0, 50ºC for 48hours. The results showed that it could hydrolyze rice husk better than corn hull, corn cob, rice straw andbagasse. The enzymatic products of rice husk were xylose and xylotetraose while the hydrolysis of cornhull, corn cob, rice straw and bagasse yielded only xylotetraose. Xylotetraose is a high valuable productand can be used in many manufacturers.

Keywords : Agricultural residue / Anoxybacillus sp. / Thermophilic bacterium / Xylanolyticenzyme / Xylotetraose

Article Details

Issue
Vol. 32 No. 1 (2009): January - March
Section
Original Articles
Author Biographies

Jitladda Thitikorn-amorn, King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150

Graduate Student, Division of Biochemical Technology, School of Bioresources and Technology.

Khin Lay Kyu, King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150

Assistant Professor, Division of Biochemical Technology, School of Bioresources and Technology.

Khanok Ratanakhanokchai, King Mongkut's University of Technology Thonburi, Takham, Bangkhuntien, Bangkok 10150

Associate Professor, Division of Biochemical Technology, School of Bioresources and Technology.

Yun-Sik Lee, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, South Korea

Lecturer, Department of Medical Zoology, College of Medicine.