Fermented Products of Bacillus sp. Strain TW-1 Grown on Corn Hull Medium under Limited Oxygen Condition

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Thanawan Srinang
Chakrit Tachaapaikoon
Khin Lay Kyu
Khanok Ratanakhanokchai
Yun-Sik Lee

Abstract

Bacillus sp. strain TW-1, grown on corn hull as a carbon source under limited oxygen conditionproduced xylanolytic enzyme (xylanase, β-xylosidase and arabinofuranosidase), cellulolytic enzyme(avicelase, carboxymethyl cellulase and β-glucosidase) and mannanase. These enzymes could hydrolyzexylan and cellulose in corn hull to xylose, xylobiose, and oligosaccharides under batch fermentation.Moreover, ethanol and acetic acid were also detected in the medium. Crude xylanolytic and cellulolyticenzymes produced by Bacillus sp. strain TW-1 could hydrolyze agricultural residues. The result showedthat the crude enzyme could be hydrolyzed corn hull better than corn cob, rice bran, sugarcane bagasseand rice straw respectively.

Keywords : Agricultural Residue / Bacillus sp. Strain TW-1 / Corn Hull /Xylanolytic and Cellulolytic Enzymes

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Original Articles
Author Biographies

Thanawan Srinang, King Mongkut's University of Technology Thonburi, Thakham, Bangkhuntien, Bangkok 10150

Graduate Student, Division of Biochemical Technology, School of Bioresources and Technology.

Chakrit Tachaapaikoon, King Mongkut's University of Technology Thonburi, Thakham, Bangkhuntien, Bangkok 10150

Researcher, EnzymeTechnology Laboratory, School of Bioresources and Technology.

Khin Lay Kyu, King Mongkut's University of Technology Thonburi, Thakham, Bangkhuntien, Bangkok 10150

Assistant Professor, Division of Biochemical Technology, School of Bioresources and Technology.

Khanok Ratanakhanokchai, King Mongkut's University of Technology Thonburi, Thakham, Bangkhuntien, Bangkok 10150

Associate Professor, Division of Biochemical Technology, School of Bioresources and Technology.

Yun-Sik Lee, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, South Korea

Lecturer, Department of Medical Zoology, College of Medicine.