Specificity of Purified Xylanase from Alkaliphilic Bacillus halodurans Strain C-1 on Hydrolysis of Soluble Xylans

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Chakrit Tachaapaikoon
King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140
Khin Lay Kyu
King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140
Khanok Ratanakhanokchai
King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140

Abstract

An extracellular xylanase from alkaliphilic Bacillus halodurans strain C-1 was purified to homogeneity, asdemonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and active-PAGE, bymeans of ammonium sulfate precipitation, DEAE-Toyopearl column, and DEAE-HiPrep FF 16/10 and Mono-QHR 5/5 fast performance liquid chromatographies. The molecular weight of the purified xylanase was estimatedto be about 47 kDa. The hydrolysis rate of soluble birchwood xylan by the purified enzyme was higher than thoseof soluble oat spelt and larchwood xylans. The Km value of the enzyme is 1.63, 1.83 and 2.76 mg/ml, againstsoluble birchwood, oat spelt and larchwood xylans, respectively, while the Vmax value of the enzyme is 0.175, 0.150and 0.125 mMmin-1/mg, respectively. The Vmax/Km ratio of xylanase on soluble birchwood xylan was approximately1.3 and 2.4 fold higher than those of soluble oat spelt and larchwood xylans, respectively.

Keywords : Alkaliphilic Bacillus halodurans strain C-1 / Purified xylanase /Specificity of xylanase / Soluble xylan

Article Details

Issue
Vol. 27 No. 1 (2004): January - March
Section
Original Articles
Author Biographies

Chakrit Tachaapaikoon, King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140

Graduate Student, Division of Biochemical Technology, School of Bioresources and Technology.

Khin Lay Kyu, King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140

Expert, Division of Biochemical Technology, School of Bioresources and Technology.

Khanok Ratanakhanokchai, King Mongkut's University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140

Associate Professor, Division of Biochemical Technology, School of Bioresources and Technology.