Effects of Types and Content of Agricultural Wastes for Oligosaccharide Production by Crude Enzyme from Penicillium oxalicum KUB-SN2-1

Abstract

Abstract

Extracellular crude enzyme from Penicillium oxalicum KUB-SN2-1 composed of cellulase, xylanase and mannanase at 2.82 ± 0.17 (carboxymethylcellulose as substrate), 1.50 ± 0.04 (birch wood xylan as substrate) and 36.01 ± 0.13 (locust bean gum as substrate) Unit/ml, respectively. Five agricultural wastes (AWs) viz. coffee residues, bagasse, soybean meal, copra meal and potato peel were selected for use as substrates for oligosaccharide production. Extracellular crude enzyme was incubated with each type of AWs, which was dissolved in 10 mM citrate-phosphate buffer pH 4.0 at 60°C for 60 h. The highest reducing sugar content of 7.51 ± 0.52 mg/ml was obtained from copra meal hydrolysate after 30 h. The reducing sugar contents from the hydrolysates of coffee residues, potato peel, bagasse and soybean meal were 3.35 ± 0.16 (48 h), 3.31 ± 1.43 (48 h), 4.10 ± 0.40 (48 h) and 2.32 ± 0.15 (48 h) mg/ml, respectively. The optimal concentration of AWs was also investigated. The maximum reducing sugar contents were given by 10% of copra meal, coffee residues, potato peel and bagasse and 8% of soy bean meal. Then, hydrolysate products were analyzed. Mannose was detected as the soybean meal hydrolysate product. Glucose was found as the bagasse and potato peel hydrolysate products, while, unknown sugars were identified as the copra meal and coffee residues hydrolysate products.

Keywords : Lignocellulase ; Oligosaccharide ; Penicillium oxalicum