Genetic Relationship Assessment and Identification of Orchids in the Genus Eria Using HAT-RAPD Markers

Authors

  • Jutamas Jiemjuejun Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Somchit Damrianant Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Theerachai Thanananta Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Narumol Thanananta Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under Royal Patronage, Pathum Thani 13180, Thailand

Keywords:

Eria, Orchids, HAT-RAPD markers, Genetic relationships

Abstract

High Annealing Temperature-Random Amplified Polymorphic DNA (HAT-RAPD) markers were used to study the genetic relationship among 15 samples of orchids in the genus Eria. DNA amplification of 31 random primers out of 72 random primers was successful. In this study, we selected 21 random primers with clear amplified products for further genetic analysis. The result showed significant differences among all samples and also showed specific bands in each sample. Moreover, we found 21 random primers which tended to be DNA markers for further identification. Based on polymorphic bands, a dendrogram was constructed. Genetic similarities among 15 samples of Eria species were demonstrated, and the samples could be classified into 3 groups with similarity coefficients ranging from 0.36 to 0.85.

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Published

2017-12-01

How to Cite

Jiemjuejun, J., Damrianant, S., Thanananta, T., & Thanananta, N. (2017). Genetic Relationship Assessment and Identification of Orchids in the Genus Eria Using HAT-RAPD Markers. Science & Technology Asia, 22(4), 19–26. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/110508

Issue

Section

Biological sciences