Validated High Performance Liquid Chromatographic (HPLC) Method for Anti-inflammation Activity of Lom-Am-Ma-Preuk Remedy

  • Krit Piwngam Department of Applied Thai Traditional Medicine Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Puritat Kanokkangsadal Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand, Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Weerachai Pipatrattanaseree Regional Medical Science Center 12 Songkhla, Department of Medical Sciences, Songkhla 90100, Thailand
  • Rodsarin Yamprasert Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Chadchom Chookong Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Buncha Ooraikul Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand, Department of Agricultural food and Nutritional Science, Faculty of Agricultural Life and Environmental Sciences, University of Alberta, Edmonton T6G2R3, Canada
  • Arunporn Itharat Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand, Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
Keywords: Method validation, HPLC, Eugenol, Myristicin, Piperine, Lom-Am-Ma-Preuk

Abstract

Lom-Am-Ma-Preuk (LAP) remedy is a Thai traditional medicine procedure for muscle pain relief. Active ingredients from LAP remedy are eugenol, myristicin and piperine. These standard markers also have been proven to have anti-inflammatory activity. The aim of this study was to develop a validated HPLC method for determination of markers in the LAP remedy to assess the validation parameters. Results show that the validation parameters were specificity, limit of detection, limit of quantification, linearity, accuracy and precision. The developed method was specific to these markers with the retention times of 45.7, 56.6 and 53.9 min for eugenol, myristicin and piperine, respectively. The coefficient of determination (R2) was more than 0.999. The accuracy and precision were in an acceptable range with good recovery verified by coefficient of variation of less than 2%. The limits of detection (LOD) of eugenol, myristicin and piperine were 0.5, 0.2 and 1.3 μg/mL and limits of quantitation (LOQ) were 1.9, 0.6 and 5 μg/mL, respectively. Total analysis time was approximately 70 min. The contents of eugenol,myristicin and piperine in LAP could be obtained in one analysis for the first time. The present method is useful for routine quality control of the LAP remedy.

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Author Biographies

Krit Piwngam, Department of Applied Thai Traditional Medicine Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand

 

 

Puritat Kanokkangsadal, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand, Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand

Puritat is the second author.

Published
2020-09-21
How to Cite
Piwngam, K., Kanokkangsadal, P., Pipatrattanaseree, W., Yamprasert, R., Chookong, C., Ooraikul, B., & Itharat, A. (2020). Validated High Performance Liquid Chromatographic (HPLC) Method for Anti-inflammation Activity of Lom-Am-Ma-Preuk Remedy. Science & Technology Asia, 25(3), 60-67. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/222837