Antibacterial Activity of Mahajak Remedy and Plant Ingredients Antibacterial activity of Mahajak remedy

Abstract

Mahajak (MH) oil is a remedy in Tamra-Osodpranarai. It is used for relieving pain, and skin infection as a Thai traditional medicine. This study investigated the efficacy of the MH remedy and plant ingredient extract on antibacterial activities. The MH remedy was macerated with sesame oil, 95% ethanol, hexane, and its plant ingredients were macerated with 95% ethanol. Then, all extracts were concentrated by rotary evaporator. The extracts were investigated on antibacterial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, and Bacillus subtilis ATCC 6633 by disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results show that the 95% ethanol extract of MH remedy was the best extract of antibacterial testing. Using the disc containing 10 μL (concentrate 500 mg/mL of the extracts), the inhibition zone was observed against S. aureus (9.67±0.33 mm). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 2.50 mg/mL were obtained for residue hexane. Hexane extracts showed MIC and MBC of S.aureus and B.subtilis with similar values to 95% ethanol. However, all of the tests had no inhibitory activity against P.aeruginosa. In conclusion, this study demonstrated that 95% ethanol extracts of the MH remedy have antibacterial activity and may be a potential treatment in cases of skin infection.