Isolation and HPLC Analysis of Astilbin in Lysiphyllum strychnifolium (syn. Bauhinia strychnifolia) Stems

  • Yupaporn Sampaopan College of Oriental Medicine, Rangsit University, Pathum Thani 12000, Thailand
  • Nirawan Kitprapiumpon College of Oriental Medicine, Rangsit University, Pathum Thani 12000, Thailand
  • Sumet Kongkiatpaiboon Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University, Pathum Thani 12120, Thailand
  • Nongnaphat Duangdee Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University, Pathum Thani 12120, Thailand
  • Surapote Wongyai College of Oriental Medicine, Rangsit University, Pathum Thani 12000, Thailand
Keywords: Bauhinia strychnifolia, Lysiphyllum strychnifolium, Chemical marker, Astilbin, HPLC, Quality control

Abstract

Lysiphyllum strychnifolium (syn. Bauhinia strychnifolia), vernacularly known in Thai as “Ya Nang Daeng”, has been traditionally used for detoxification and as an anti fever in Thai traditional medicine. Despite published research on the plant’s phytochemical constituents and their anti cancer activity, quality assessment and pharmacological investigations have been hampered since the major bioactive compound remains unknown. Therefore, in this study, the isolation of a major compound has been explored, and its structure has been elucidated as astilbin. An RP HPLC method was developed and validated for the determination of astilbin in L. strychnifolium stems. The chromatographic separation was performed on a reversed phase C 18 column using gradient system of 2% (v/v) acetic acid and acetonitrile with flow rate of 1 mL·min 1. The UV detection was set at a wavelength of 290 nm. The validated method showed good sensitivity, linearity, precision, and accuracy. The method could be used for routine analysis of astilbin in L. strychnifolium stems going forward. The present study provided a useful basis for the quality control of crude plant material, extract, and phytopharmaceutical products.

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Published
2021-03-16
How to Cite
Sampaopan, Y., Kitprapiumpon, N., Kongkiatpaiboon, S., Duangdee, N., & Wongyai, S. (2021). Isolation and HPLC Analysis of Astilbin in Lysiphyllum strychnifolium (syn. Bauhinia strychnifolia) Stems. Science & Technology Asia, 26(1), 208-215. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/225205
Section
Biological sciences