Morphological and Molecular Identification of Pythium spp. from Hydroponically-Grown Lettuce

Authors

  • Chulalak Talubnak Department of Biotechnology in Plant Pathology, International College, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
  • Henk-jan Schoonbeek Department of Crop Genetics, Norwich Research Park, Norwich, NR4 7UH, UK
  • Nonglak Parinthawong Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
  • Tanimnun Jaenaksorn Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand

Keywords:

Cytochrome oxidase gene, Hydroponics, ITS region, Pythium root rot, Pathogenicity

Abstract

Herein, hydroponic Pythium communities in Thailand were investigated. The 38 Pythium isolates from asymptomatic and symptomatic lettuce roots were identified using morphological and molecular features. The data indicated that P. aphanidermatum and P. myriotylum were the predominant species. Regarding the rDNA-ITS study, all isolates were identified as P. aphanidermatum, P. myriotylum, P. deliense, or an unidentified Pythium species. The reconfirmation of the three unidentified Pythium isolates using the cytochrome oxidase subunit I (COI) gene in comparison to the representative isolates was achieved using P. aphanidermatum and P. myriotylum. The COI phylogenetic trees were similar to that of the ITS tree. Addi-tionally, pathogenicity of the Pythium representative isolates to plant seeds was evaluated in a laboratory assay. The seedlings suffered serious symptoms from P. myriotylum SR31 infection, with 90-100% disease severity. The other isolates presented disease severity of less than 20% when compared with uninoculated control. This study provides a comprehensive identification of Pythium root rot in lettuce grown on hydroponics in Thailand and provides information on beneficial microorganisms and a resistance inducer in lettuce root rot.

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Published

2022-03-28

How to Cite

Talubnak, C., Schoonbeek, H.- jan, Parinthawong, N., & Jaenaksorn, T. (2022). Morphological and Molecular Identification of Pythium spp. from Hydroponically-Grown Lettuce. Science & Technology Asia, 27(1), 143–154. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/242341

Issue

Section

Biological sciences