Development of a Serological Dilution Microfluidic Chip for Immunoassay Applications

Authors

  • Therdthai Thienthong Mechanical Engineering Simulation and Design Group, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand
  • Ekachai Juntasaro Mechanical Engineering Simulation and Design Group, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand
  • Numfon Khemthongcharoen Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom 73170, Thailand
  • Witsaroot Sripumkhai Thai Microelectronics Center, National Electronics and Computer Technology Center, National Science and Technology Development Agency, Chachoengsao 24000, Thailand
  • Nongluck Houngkamhang College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
  • Pattaraluck Pattamang Thai Microelectronics Center, National Electronics and Computer Technology Center, National Science and Technology Development Agency, Chachoengsao 24000, Thailand
  • Mayuree Chanasakulniyom Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand
  • Nithi Atthi Thai Microelectronics Center, National Electronics and Computer Technology Center, National Science and Technology Development Agency, Chachoengsao 24000, Thailand
  • Chamras Promptmas Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom 73170, Thailand
  • Panapat Uawithya Department of Physiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
  • Wutthinan Jeamsaksiri Thai Microelectronics Center, National Electronics and Computer Technology Center, National Science and Technology Development Agency, Chachoengsao 24000, Thailand

Keywords:

Dilution, Human serum, Microfluidics, Micromixer, Volume flow rate control network

Abstract

This work aims to develop a multiple dilution microfluidic chip that is capable of diluting the human serum by means of two-fold dilution for seven levels from 1:2 to 1:128 with phosphate-buffered saline (PBS) buffer. The dilution in this work is processed in parallel in order to reduce the accumulated errors that the standard pipetting technique generates in the micro-well plate. The serum and PBS buffer are precisely delivered to the micromixers by controlling their flow rates. The dilution is achieved by the passive mixing process for which the serpentine geometry is designed in order to continually generate the Dean vortices along the serpentine microchannel to effectively mix serum and PBS buffer in the microfluidic chip. The prototype of this multiple dilution microfluidic chip is fabricated by using polydimethylsiloxane (PDMS). The dilution-in-parallel capability of this prototype is validated by using the UV-vis absorption method. The results reveal that the measured values of the seven dilution ratios obtained are in good agreement with the exact values. Finally, this prototype is evaluated for serological MOG-IgG detection in order to verify the reliable operation of this multiple dilution microfluidic chip. The prototype can successfully detect MOG-IgG at all volume concentration ratios.

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Published

2022-09-28

How to Cite

Therdthai Thienthong, Ekachai Juntasaro, Numfon Khemthongcharoen, Witsaroot Sripumkhai, Nongluck Houngkamhang, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Nithi Atthi, Chamras Promptmas, Panapat Uawithya, & Wutthinan Jeamsaksiri. (2022). Development of a Serological Dilution Microfluidic Chip for Immunoassay Applications. Science & Technology Asia, 27(3), 152–174. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/247445

Issue

Section

Engineering