Prevalence of Extended-Spectrum β-Lactamase-producing Enterobacterales and Distribution of blaESBL Genes from Patients who Underwent Abdominal Surgery

Authors

  • Sumalee Kondo Division of Molecular Genetics and Molecular Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Anucha Apisarnthanarak Division of Infectious Diseases, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Suwanna Trakulsomboon Faculty of Medical Technology, Nakhonratchasima College, Nakhonratchasima 30000, Thailand
  • Wannapa Bootkotr Research administration office, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Chatchai Mingmalairak Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Prasit Mahawongkajit Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Jatupong Juntong Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Palin Limpavitayaporn Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Ekkapak Sriussadaporn Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Assanee Tongyoo Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Piyapong boonyasatid Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Trirat Chunsirisub Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Krisada Nakornchai Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Weerayut Thowprasert Department of Surgery, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand
  • Pattarachai Kiratisin Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
  • Prasit Palittapongarnpim Pornchai Matangkasumbut Center for Microbial Genomics, Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10700, Thailand

Keywords:

Abdominal surgery, blaESBL genes, Colonization, ESBL producing E. coli, ESBL producing K. pneumoniae, Extended-spectrum β-lactamase (ESBL)-producing Enterobacterales, Rectal swabs

Abstract

The proliferation of extended-spectrum β-lactamase-producing Enterobacterales (ESBLPE) is a serious global health problem. Screening for ESBL-PE from rectal swabs of patients who underwent abdominal surgery and determination of blaESBL genes were performed. E. coli producing the extended-spectrum β-lactamase enzyme (ESBL) (77.41%) and ESBL-producing K. pneumoniae (12.9%) were found in 31 out of 104 patients. The most prevalent blaESBL in both E. coli and K. pneumoniae were blaTEM and blaOXA-2, whereas blaSHV was most prevalent only among K. pneumoniae. In contrast, blaCTX-M and blaOXA-10 were the least detected in E. coli and K. pneumoniae. The fecal ESBL producers isolated from swabs taken prior to and after abdominal surgery illustrated persistence of colonization in patients’ guts. Multiple- drug resistant bacterial infections found among ESBL carriages appeared to be associated with prolonged hospitalization and underlying complex diseases. It is essential that healthcare professionals are cognizant of the importance of monitoring for the presence of these ESBL producers, and reducing both intra-abdominal surgery site infections and the spread of resistance genes in prolonged fecal ESBL carriages in high-risk patients.

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Published

2022-12-31

How to Cite

Sumalee Kondo, Anucha Apisarnthanarak, Suwanna Trakulsomboon, Wannapa Bootkotr, Chatchai Mingmalairak, Prasit Mahawongkajit, Jatupong Juntong, Palin Limpavitayaporn, Ekkapak Sriussadaporn, Assanee Tongyoo, Piyapong boonyasatid, Trirat Chunsirisub, Krisada Nakornchai, Weerayut Thowprasert, Pattarachai Kiratisin, & Prasit Palittapongarnpim. (2022). Prevalence of Extended-Spectrum β-Lactamase-producing Enterobacterales and Distribution of blaESBL Genes from Patients who Underwent Abdominal Surgery. Science & Technology Asia, 27(4), 104–116. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/248055

Issue

Section

Biological sciences