Genetic Distance and Conservation Status of Stingray Species at TPI Tasik Agung Rembang, Central Java based on the Mitochondrial Cytochrome Oxidase I Gene
Article Sidebar
Main Article Content
Abstract
Genetic distance and conservation status of stingray species at TPI Tasik Agung Rembang, Central Java utilizes the mitochondrial cytochrome oxidase I (COI) gene. The objective of this research is to examine the genetic distance and conservation status of five stingray species discovered in TPI (Fish Auction) Tasik Agung: Himantura uarnacoides, Himantura walga, Himantura gerrardi, Rhinobatos penggali, and Brevitrygon heterura. Eventually, the acquired data can be applied as important basic information to preserve genetic diversity in stingray populations. The DNA barcode approach was performed on the COI gene using the polymerase chain reaction (PCR) technique. The sequences obtained were edited in the Basic Local Alignment Search Tool (BLAST) program before being analyzed in MEGA 10 software for phylogenetic tree reconstruction and genetic distance measurement. The genetic distance between the most closely related species was 0.002 (Dasyatis zugei and Telatrygon zugei), and the most significant genetic distance was 0.231 (Rhinobatos penggali and Brevitrygon heterura with Himantura walga). The IUCN conservation status of stingrays observed during the study revealed four species in the vulnerable category (VU) and one species in the near threatened (NT) category.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Kementerian Kelautan dan Perikanan Republik Indonesia. 2018. Kementerian Produktivitas Perikanan Indonesia. Jakarta: https://kkp.go.id/wp
Badan Pusat Statistik Provinsi Jawa Tengah. 2020. Produksi Perikanan Tangkap Laut Menurut Kabupaten di Jawa Tengah Tahun 2016 2019. Semarang: Badan Pusat Statistik.https://jateng.bps.go.id/subject/56/perikanan.html
Utami, MNS, Sri Redjeki, Nur Taufiq SPJ. 2014. Studi Biologi Ikan Pari (Dasyatis sp) di TPI Tasik Agung Rembang. Journal Of Marine Research. Volume 2, Nomor 3, Halaman 79-85 Online di: http://ejournals1.undip.ac.id/index.php/jmr
Zain BMM, et al., 2018. What is an‘elasmobranch’? The impact of palaeontology in understanding elasmobranch phylogeny and evolution https://doi.org/10.1111/j.1095-8649.2012.03245.x
Setiati, N., Indriyanti DR, Partaya. 2020. Status Kepunahan dan Upaya Konservasi Jenis-jenis Ikan Chondrichtyes yang Teridentifikasi di TPI Tegalsari, Kota Tegal. Vol. 5 (1): 34-41.
Setiati, N., Partaya, Hidayah N., 2020. The use of two pairs primer for CO1 gene amplification on traded stingray at fish auction Tasik Agung Rembang. Journal of Physics: Conference Series 1567, 1-6.
Setiati, N., Peniati, E., Partaya. Diversity and Conservation Knowledge of Chondrichthyans in Northern Coastal Areas of Central Java. 2018 Biosaintifika. 10(2). P.471-6.
Last, PR, Naylor GJP, Manjaji MBM. 2016a. A revised classification of the family Dasyatidae (Chondrichthyes: Myliobatiformes) based on new morphological and molecular in-sights. Zootaxa,4139: 345-68.
John BA, Sheikh HI, Jalal K, Zaleha K, Kamaruzzaman B (2018) DNA Barcoding of Rays from the South China Sea. Springer, Switzer land, pp 121-36.
International Union for Conservation of Nature. 2015. IUCN Red List ofThreatenedSpecies. http://www.iucnredlist.org/
Le Port, M. D. M. Pawley, S. D. Lavery. 2013. Speciation of two stingrays with antitropical distributions: low levels of divergence in mitochondrial DNA and morphological characters suggest recent evolution. Aquatic Biology 19: 153-65.
Aisyah S, Risna H, Okto S, Ahmad FS. 2020. DNA Barcoding dan Status Konservasi Ikan Hiu (Hemiscylliidae dan Charcharhinidae) yang didaratkan di PPN Sungailiat Bangka. Journal of Fisheries and Marine Research. pp. 316-23.
Bineesh K, Gopalakrishnan A, Akhilesh K, Sajeela K, Abdussamad E, Pillai N. 2016 DNA barcoding reveals species composition of sharks and rays in the Indian commercial fishery. Mitochondrial DNA A DNA Mapp Seq Anal 28(4):115.
Chen 2016 Complete mitochondrial genome of the Sharpnose stingray Himantura gerrardi (Myliobatiformes: Dasyatidae) Mitochondrial DNA Online ISSN: 1940-1744 DOI:10.3109/19401736.2014.989518
Wulandari, Merlia TN and Rai HA. 2021. Study Identification of Some Species of Fish Using the Partial Fragment of Mitochondrial Cytochrome Oxidase Subunit-1Gene (COI) in Danau Panggang, South Borneo. Journal of Aquaculture and Fish Health 10(2) : 229-38.
Mufti S, Khairiza L. Achmad Farajallah, Nisfa Hani. 2020. Short Communication: The mitochondrial cytochrome c oxidase subunit I (COI) for identification of batoids collected from landed sites in Medan, Indonesia. Biodiversitas. 21 (11) : 5414-21.
Ruocco NL, Lucifora LO, de Astarloa JMD, Mabragana E, Delpiani SM. (2012). Morphology and DNA barcoding reveal a new species of eagle ray from the southwestern Atlantic: Myliobatis ridens sp. nov. (Chondrichthyes: Myliobatiformes: Myliobatidae). Zool Stud 51:862-73.
Madduppa H, Ayuningtyas RU, Subhan B, Arafat D. 2016. Exploited but unevaluated: DNA barcoding reveals skates and stingrays (Chordata, Chondrichthyes) species landed in the Indonesian fish market. ILMU KELAUTAN: Indonesian J Marine Sci 21(2):77–84.
Rahayu, D. A., Nugroho, E. D., & Listtyorini, D. 2019. DNA Barcoding Ikan Introduksi Khas Telaga Sari, Kabupaten Pasuruan. Biotropika: Journal of Tropical Biology, 7(2).
Kar A, Raut SK, Bhattacharya S, Patra S. & Basanta. 2017. Marine fishes of West Bengal coast, India: Diversity and conservation preclusion. Regional Studies in Marine Science. 16. DOI:10.1016/J.RSMA.2017.08.009.
Fotedar, Lukehurst, Jackson, Snow. 2019. Molecular tools for identification of shark species involved in depredation incidents in Western Australian fisheries. PLOS ONE, 14(1), e0210500.
Pawan-Kumar A, Gireesh-Babu P, Suresh Babu PP, Jaiswar AK, Pani Prasad K, Aparna Chaudhari S, Raje SG. 2015. DNA barcoding of elasmo-branchs from Indian Coast and its reliability in delineating geographically widespread specimens. Mitochondrial DNA 26:1-9.
Setiati, N., Indriyanti DR, Partaya. 2020. Status Kepunahan dan Upaya Konservasi Jenis-jenis Ikan Chondrichtyes yang Teridentifikasi di TPI Tegalsari, Kota Tegal. Vol. 5 (1): 34-41.
Kumar S, Glen S, Michael L, Christina K, Koichiro Tamura, 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Journal Molecular Biology and Evolution, (35) 6, Pages 1547-9.
Berthe C. & Lecchini. 2016. Influence of boat noises on escape behaviour of whitespotted eagle ray Aetobatus ocellatus at Moorea Island (French Polynesia). Comptet Rendus Biologies 339.
Yuwandana DP, Agustina S, Haqqi MB, Simeon BM. 2020. Studi Awal Perikanan Pari Kekeh (Rhynchobatus sp.) dan Pari Kikir (Glaucostegus sp.) di Perairan Utara Jawa Tengah. Jurnal Akuatika Indonesia. 5(1).
Puckridge M, Last PR, White WT & Andreakis N. 2013. Phylogeography of the Indo‐West Pacific maskrays (Dasyatidae, Neotrygon): A complex example of chondrichthyan radiation in the Cenozoic. Ecology and Evolution, 3(2), 217-32.
Setiati,N., Novita A.L, Partaya, Bambang P. 2020. Kajian aspek biologi dan status kepunahan ikan pari yang diperdagangkan di TPI pantai utara Jawa Tengah. Proseding Seminar Nasional Pendidikan Biologi dan Saintek (SNPBS) ke-V. p-ISSN: 2527-533X. pp: 690-8.
Arlyza K, Shen, Solihin DD, Berrebi P, & Borsa. 2013. Species boundaries in the Himantura uarnak species complex (Myliobatiformes: Dasyatidae). Molecular Phylogenetics and Evolution 66. DOI: 10.1016/j.ympev.2012.09.023.