Cell Shape Description as a Tool for Assessing Cell Change after Drug Treatments
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Abstract
Cell shape determines cell activities and behavior. Quantitative analysis of cell shape can be performed by computational image-based analysis. Therefore, the aim of this study was to characterize cell-shape change using various cell-shape descriptions. Pre-osteoblasts were treated with the osteoporosis drugs, alendronate and geranylgeraniol. Cell shapes were defined via ImageJ analysis. Parameters, including perimeter, area/perimeter, circularity, roundness and solidity, were quantified. Alendronate and geranylgeraniol treatments generated changes in cell shape, which were compared between control cells and treated cells. Cell perimeter was increased when
geranylgeraniol was added to alendronate-treated cells. Area/perimeter and solidity showed the same trend after treatments. Alendronate decreased both area/perimeter and solidity, while geranylgeraniol increased these parameters toward the baseline level. Circularity was reduced by alendronate and geranylgeraniol. No difference in roundness was observed in either alendronate or geranylgeraniol treatments. Taken together, these parameter measurements demonstrated changes in the cell shape and the cell surface. Each parameter gave distinctive and overlapping information on cell-shape analysis. Evaluation of cells using combined parameters provided a better insight into the cell changes. These data could be applied for the screening of cell changes after treating
with drugs.
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