The Effect of Probiotic Fermented Milk on Salivary Streptococcus mutans count

Authors

  • Darunee Choomroy นิสิตหลักสูตรวิทยาศาสตรมหาบัณฑิต สาขาวิชาทันตกรรมทั่วไปขั้นสูง คณะทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ
  • Bhornsawan Thanathornwong รองศาสตราจารย์ ภาควิชาทันตกรรมทั่วไปขั้นสูง คณะทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ
  • Suteera Techatanawat อาจารย์ ภาควิชาทันตกรรมทั่วไปขั้นสูง คณะทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ
  • Pimsupak Boonmongkolraksa อาจารย์ ภาควิชาทันตกรรมทั่วไปขั้นสูง คณะทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ

Keywords:

Probiotic fermented milk, Salivary Streptococcus mutans count, Lactobacillus paracasei

Abstract

Objective: To investigate the effects of probiotic milk containing Lactobacillus paracasei on Streptococcus mutans (S. mutans) counts in saliva. Subjects and methods: Forty participants were recruited in this single-blinded, randomized and controlled crossover study. During the intervention periods, the participants were asked to drink 15 ml of either probiotic milk or control water after breakfast every day for 10 days. The pre- and post- intervention salivary samples were subjected to microbiological evaluation. The numbers of S. mutans were determined by plating counting, the bacteria were identified by colony morphology. Results: There was a significant reduction in the S. mutans count after drinking probiotic milk (p<0.001). On the contrary, no significant change in the S. mutans count between the baseline and after drinking water. Moreover, the S. mutans count after probiotic milk consumption was significantly lower than after water consumption (p<0.001). There were no significant differences in the S. mutans count between probiotic milk and water consumption at the baseline. Conclusion: The daily consumption of milk containing probiotic bacteria can reduce levels of S. mutans and may contribute to the prevention of dental caries.

References

Caufield PW, Griffen AL. Dental caries: an infectious and transmissible disease. Pediatric Clinics of North America. 2000;47(5):1001-1019.

Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. Journal of dental education. 2001;65(10):1028-1037.

Kuramitsu HK. Virulence properties of oral bacteria: impact of molecular biology. Current issues in molecular biology. 2001;3(2):35-36.

Tinanoff N, Kanellis MJ, Vargas C. Current understanding of the epidemiology, mechanisms, and prevention of dental caries in preschool children. Pediatric dentistry. 2002;24(6):543-551.

Ganguly N, Bhattacharya S, Sesikeran B, Nair G, Ramakrishna B, Sachdev H, et al. ICMR-DBT guidelines for evaluation of probiotics in food. The Indian journal of medical research. 2011;134(1):22.

Näse L, Hatakka K, Savilahti E, Saxelin M, Pönkä A, Poussa T, et al. Effect of long–term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries research. 2001;35(6):412-420.

Holz C, Alexander C, Balcke C, Moré M, Auinger A, Bauer M, et al. Lactobacillus paracasei DSMZ16671 reduces mutans streptococci: a short-term pilot study. Probiotics and antimicrobial proteins. 2013;5(4):259-263.

Sookkhee S, Chulasiri M, Prachyabrued W. Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens. Journal of applied microbiology. 2001;90(2):172-179.

Chuang L-C, Huang C-S, Ou-Yang L-W, Lin S-Y. Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora. Clinical oral investigations. 2011;15(4):471-476.

Teanpaisan R, Piwat S, Dahlen G. Inhibitory effect of oral Lactobacillus against oral pathogens. Letters in applied microbiology. 2011;53(4):452-459.

Teanpaisan R, Piwat S. Lactobacillus paracasei SD1, a novel probiotic, reduces mutans streptococci in human volunteers: a randomized placebo-controlled trial. Clinical oral investigations. 2014;18(3):857-862.

Teanpaisan R, Piwat S, Tianviwat S, Sophatha B, Kampoo T. Effect of long-term consumption of Lactobacillus paracasei SD1 on reducing mutans streptococci and caries risk: a randomized placebo-controlled trial. Dentistry journal. 2015;3(2):43-54.

Levine R. Briefing paper: Milk, flavoured milk products and caries. British dental journal. 2001;191(1): 120.

Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and remineralization (part 1). Journal of Clinical Pediatric Dentistry. 2004;28(1):47-52.

Smith DJ, Taubman MA, Ebersole JL. Salivary IgA antibody to glucosyltransferase in man. Clin Exp Immunol. 1985;61 :416-424.

Emilson CG. Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva. Scand J Dent Res. 1983; 91(1): 26-32.

Downloads

Published

2021-10-16

Issue

Section

บทความวิจัย