Macroscopic, Microscopic and HPLC Chromatogram Studies of the Crude Drug Called “Chetta Phang Khi”


  • Phonphatcharin Nonthasin นักศึกษา หลักสูตรวิทยาศาสตรมหาบัณฑิต สาขาวิชาเภสัชกรรมแผนไทย คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น
  • Tanit Padumanonda ผู้ช่วยศาสตราจารย์ ภาควิชาเภสัชเวทและพิษวิทยา คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น
  • Prathan luecha รองศาสตราจารย์ ภาควิชาเภสัชเวทและพิษวิทยา คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น
  • Suppachai Tiyaworanant ผู้ช่วยศาสตราจารย์ ภาควิชาเภสัชเวทและพิษวิทยา คณะเภสัชศาสตร์ มหาวิทยาลัยขอนแก่น


Chetta Phang Khi, Macroscopical, Microscopical, HPLC chromatograms


“Chetta Phang Khi” or “ Phang Khi” are the name of a plant that is used as an ingredient in several medicinal recipes. In particular, Pasa Chetta Phang Khi has been included in Thailand list of essential medicines. This recipe contains 50 percent of Chetta Phang Khi crude drug. According to the literature search, either Chetta Phang Khi or Phang Khi are mentioned and classified in the family of Euphorbiaceae with the scientific names Croton crassifolius Geiseler and Cladogynos orientalis Zipp. ex Span. This is the cause of confusion regarding the utilization and the reference of this crude drug in the the other research or the existing research. Therefore, it is necessary to study the macroscopical and microscopical characters as well as the study on the HPLC chromatograms of crude drugs from the authentic samples (root) of both species as well as the commercial sources. The results showed the statistical difference in the average ratio of xylem thickness to cortex thickness between C. crassifolius and C. orientalis (0.689 ±0.107 and 10.268 ±1.979, respectively; p < 0.05). Samples from the commercial sources exhibited in the average ratio of xylem thickness to cortex thickness ranged from 0.532±0.026 to 1.061±0.049 which were not statistically different from the authentic samples of C. crassifolius. The chromatogram of C. orientalis and commercial samples were different from those of C. orientalis according to the chromatographic peak eluted between 35-40 minutes which was absent in C. orientalis ‘s extracts.


Wut W. Pharmacy Rattanakosin scriptures: 71/92-93 Petchkasem Road 81/2 Nong Khang Phlu Subdistrict Nong Khaem county 10160; 2002. Thai.

Chatchai S. Guide to herbal medicines in the National List of 71 Recipes of Essential Medicines Specify the color according: Wat Mai Subdistrict, Mueang District Nonthaburi Province.: Phrapokklao Hospital Group Nonthaburi Province;. Thai.

herbal database Faculty of Pharmacy Ubon Ratchathani University. [Internet] [cited 2018 Sep 18]

Available from: Thai

plant name Tem Smitinan. [Internet]. [cited 2017 Nov 18 ]. Available from:พังคี&keyback=พังคี. Thai.

N-ROYAL-INSTITUTED [Internet] [cited 2017 Sep 10]. Available from: Thai

plants database The Botanical Garden Organization. Chetta Phang Khi [Internet] [cited 2017 Sep 8] Available From: Thai.

plants database The Botanical Garden Organization. Chetta Phang Khi [Internet] [cited 2018 Sep 18] Available From: database/botanic_book%20full%20option/search_detail.asp?botanic_id=1171.Thai.

Thai medicine textbook preparation group Bureau of Drug and Narcotic. Department of Medical Sciences 2017. Thai Herbal Pharmacopoeia In the textbook of Thai herbal medicine standards: Nonthaburi: Bureau of Drug and Narcotic Department of Medical Sciences; 2017. Thai.

Chalong S. Chapter 1 Introduction to Statistics (Basic Statistics) [Internet]. 2012 [cited 2017 June 10] Available from: Thai.

Huang W, Wang J, Liang Y, Ge W, Wang G, Li Y, Chung HY. Potent anti-angiogenic component in Croton crassifolius and its mechanism of action. Journal of ethnopharmacology. 2015 Dec 4;175:185-91.

Lux A, Luxová M, Abe J, Morita S. Root cortex: structural and functional variability and responses to environmental stress. Root Research. 2004 Sep 29;13(3):117-31.