Antioxidant Activity, Total Phenolic, Flavonoid, and Tannin Contents from Phlebopus colossus (R.Heim) Singer and Boletus griseipurpureus Corner Crude Extract with Various Solvents

Authors

  • Khwanyuruan Naksuwankul รองศาสตราจารย์ ภาควิชาชีววิทยาและหน่วยวิจัยเห็ดและไลเคนส์เพื่อการใช้ประโยชน์อย่างยั่งยืน คณะวิทยาศาสตร์ มหาวิทยาลัยมหาสารคาม
  • Nucharee Chamnantap นักศึกษา หลักสูตรวิทยาศาสตรบัณฑิต สาขาชีววิทยา ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยมหาสารคาม
  • Areerat Saisong นักศึกษา หลักสูตรวิทยาศาสตรบัณฑิต สาขาชีววิทยา ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยมหาสารคาม
  • Kritsada Champatasi นักศึกษา หลักสูตรวิทยาศาสตรบัณฑิต สาขาชีววิทยา ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยมหาสารคาม

Keywords:

Antioxidant, Boletes mushroom, Polyphenol

Abstract

This study aims to evaluate antioxidant activity, to analyze total phenolic, flavonoid, and tannin contents from a crude extract of Phlebopus colossus (R. Heim) Singer and Boletus griseipurpureus Corner. The crude extracts were maceration with 2 solvents such as methanol, 95% ethanol, and with boiling water for 1 hr. The results showed higher antioxidant activities by DPPH assay with IC50 = 0.09±0.04 mg/mL from boiling water crude extract of B. griseipurpureus, ABTS assay showed higher from boiling water crude extract of P. colossus with IC50 = 1.03±0.03 mg/mL, while FRAP assay was found higher FRAP value = 3608.89±120.68 mg FeSO4/g extract from an ethanolic crude extract  of  B. griseipurpureus. Total phenolic and flavonoid contents were showed the highest value from an ethanolic crude extract from P. colossus at 34.00±0.17 mg GAE/g extract and 316.62±6.16 mg QE /g extract. Whereas, tannin content was found higher from B. griseipurpureus with boiling water extract at 14.73±0.34 mg Tannic /g extract. While, polyphenol content from boletes mushroom crude extract was found amount different, but show high valuable antioxidant properties.

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Published

2022-02-18

Issue

Vol. 23 No. 1 (2023)

Section

บทความวิจัย

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