Anti-Allergy Activity, Chemical Content and Stability Testing of Herbal Formulation Extract for Treatment of Urticaria
Keywords:
Anti-allergy activity, Urticaria, Herbal medicine, Kae-Lom-Pid formulationAbstract
The Kae-Lom-Pid formulation (KLP) is a combination of A.ilicifolius, A.ascalonicum, B.blumeana, and R.nasutus, as suggested in the therapy guidelines for Applied Thai Traditional Medicine. The study was to evaluate the in vitro anti-allergy activity of each plant and the KLP formulation extracts. It included chemical analysis and stability testing. The dried plant components were mixed and macerated in 95% ethanol for extraction. The antiallergy properties were evaluated by measuring the release of β-hexosaminidase and evaluating the levels of IL-4 and histamine release on RBL-2H3 cell lines by ELISA. The findings showed the KLP extract (HDE) effectively suppressed the activity of β-hexosaminidase, IL-4, and histamine release, with IC50 values of 87.04±1.40, 50.90±0.24, and 22.59±0.41 µg/mL, respectively. The HDE contains rhinacanthin-C and -N at a concentration of 1.10±0.02% and 0.15±0.00%w/w, respectively, determined by HPLC. Furthermore, HDE remained stable when stored at 40±2°C, 75±5% RH for a period of 6 months. The findings suggest that HDE has a tendency to alleviate allergies.
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