การสังเคราะห์พอลิไวนิลแอลกอฮอล์/ไคโตซานไฮโดรเจลคอมโพสิตที่ประกอบด้วยยาปฏิชีวนะและอนุภาคเงินระดับนาโนด้วยวิธีที่เปนมิตรต่อสิ่งแวดล้อม
คำสำคัญ:
แผ่นปิดแผล, การนำส่งยา, ไฮโดรเจลนาโนคอมโพสิตบทคัดย่อ
งานวิจัยนี้มีจุดประสงค์เพื่อพัฒนาไฮโดรเจลจากพอลิไวนิลแอลกอฮอล์ (PVA) และไคโตซาน (CS) โดยพบว่าอัตราส่วนที่เหมาะสมต่อดัชนีการบวมน้ำสูงสุดเท่ากับ 2.5:2.5 โดยน้ำหนัก จากนั้นนำอัตราส่วนดังกล่าวไปประยุกต์ใช้ร่วมกับอนุภาคเงินระดับนาโน (AgNPs) ที่สังเคราะห์ด้วยสารสกัดจากกากกาแฟ (Ex-SCG) จากผลการทดลองพบว่าเกิด AgNPs เพิ่มขึ้นตามความเข้มข้นของซิลเวอร์ไนเตรต (AgNO3) ตั้งแต่ 20-100 มิลลิโมลาร์ ไฮโดรเจลนาโนคอมโพสิตที่มี AgNO3 50 มิลลิโมลาร์ (H-AgNPs-50) แสดงค่าร้อยละดัชนีการบวมน้ำสูงสุดที่ 237.7 และมีค่าความเค้นอัด 10.07 ± 4 เมกาปาสคาล นอกจากนี้ การมีอยู่ของ AgNPs ในไฮโดรเจลช่วยเพิ่มประสิทธิภาพในการต้านเชื้อร่วมกับยาปฏิชีวนะวะ Ceftriaxone sodium โดย H-AgNPs-50 แสดงการต้านเชื้อ E. coli และ S. Aureus เพิ่มขึ้นร้อยละ 6.05 และ 19.12 ตามลำดับ เมื่อเทียบกับ H-AgNPs-0 ด้วยคุณสมบัติดังกล่าว H-AgNPs-50 อาจมีศักยภาพในการนำไปพัฒนาใช้เป็นแผ่นปิดแผลและวัสดุนำส่งยาได้
เอกสารอ้างอิง
Koosha M, Aalipour H, Shirazi MJS, Jebali A, Chi H, Hamedi S, et al. Physically crosslinked chitosan/pva hydrogels containing honey and allantoin with long-term biocompatibility for skin wound repair: An in vitro and in vivo study. J Funct Biomater. 2021; 12(4).
Suflet DM, Popescu I, Pelin IM, Ichim DL, Daraba OM, Constantin M, et al. Dual cross-linked chitosan/pva hydrogels containing silver nanoparticles with antimicrobial properties. Pharmaceutics. 2021; 13(9): 1–18.
Shamloo A, Aghababaie Z, Afjoul H, Jami M, Bidgoli MR, Vossoughi M, et al. Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study. Int J Pharm. 2021; 59: 120068. Available from: https://doi.org/10.1016/j.ijpharm.2020.120068
Massarelli E, Silva D, Pimenta AFR, Fernandes AI, Mata JLG, Armês H, et al. Polyvinyl alcohol/chitosan wound dressings loaded with antiseptics. Int J Pharm. 2021; 593: 120110.
Lima T, Passos MF. Skin wounds, the healing process, and hydrogel-based wound dressings: a short review. J Biomater Sci Polym Ed. 2021; 32(14): 1910–1925 Available from: https://doi.org/10.1080/09205063.2021.1946461
Kumar A, Behl T, Chadha S. Synthesis of physically crosslinked PVA/Chitosan loaded silver nanoparticles hydrogels with tunable mechanical properties and antibacterial effects. Int J Biol Macromol. 2020; 149: 1262–1274. Available from: https://doi.org/10.1016/j.ijbiomac.2020.02.048
Zhuang PY, Li YL, Fan L, Lin J, Hu QL. Modification of chitosan membrane with poly(vinyl alcohol) and biocompatibility evaluation. Int J Biol Macromol. 2012; 50(3): 658–663. Available from: http://dx.doi.org/10.1016/j.ijbiomac.2012.01.026
Sung JH, Hwang M-R, Kim JO, Lee JH, Kim Y Il, Kim JH, et al. Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan. Int J Pharm. 2010; 392(1): 232–240. Available from: https://www.sciencedirect.com/science/article/pii/S0378517310001985
Srikhao N, Kasemsiri P, Ounkaew A, Lorwanishpaisarn N, Okhawilai M, Pongsa U, et al. Bioactive Nanocomposite Film Based on Cassava Starch/Polyvinyl Alcohol Containing Green Synthesized Silver Nanoparticles. J Polym Environ. 2021; 29(2): 672–684. Available from: https://doi.org/10.1007/s10924-020-01909-2
Batool S, Hussain Z, Niazi MBK, Liaqat U, Afzal M. Biogenic synthesis of silver nanoparticles and evaluation of physical and antimicrobial properties of Ag/PVA/starch nanocomposites hydrogel membranes for wound dressing application. J Drug Deliv Sci Technol. 2019; 52: 403–414. Available from: https://doi.org/10.1016/j.jddst.2019.05.016
Chakravarty A, Ahmad I, Singh P, Ud Din Sheikh M, Aalam G, Sagadevan S, et al. Green synthesis of silver nanoparticles using fruits extracts of Syzygium cumini and their bioactivity. Chem Phys Lett. 2022; 795: 139493. Available from: https://www.sciencedirect.com/science/article/pii/S0009261422001609
Srikhao N, Kasemsiri P, Lorwanishpaisarn N, Okhawilai M. Green synthesis of silver nanoparticles using sugarcane leaves extract for colorimetric detection of ammonia and hydrogen peroxide. Res Chem Intermed. 2021; 47(3): 1269–1283. Available from: https://doi.org/10.1007/s11164-020-04354-x
Ounkaew A, Kasemsiri P, Srichiangsa N, Hiziroglu S, Maraphum K, Posom J, et al. Green synthesis of nanosilver coating on paper for ripening delay of fruits under visible light. J Environ Chem Eng. 2021; 9(2): 105094. Available from: https://doi.org/10.1016/j.jece.2021.105094
Taesuwan I, Ounkaew A, Okhawilai M, Hiziroglu S, Jarernboon W, Chindaprasirt P, et al. Smart conductive nanocomposite hydrogel containing green synthesized nanosilver for use in an eco-friendly strain sensor. Cellulose. 2021; 29: 273–286.
Ounkaew A, Kasemsiri P, Jetsrisuparb K, Uyama H, Hsu YI, Boonmars T, et al. Synthesis of nanocomposite hydrogel based carboxymethyl starch/polyvinyl alcohol/nanosilver for biomedical materials. Carbohydr Polym. 2020; 248: 116767.
Panzella L, Cerruti P, Aprea P, Paolillo R, Pellegrino G, Moccia F, et al. Silver nanoparticles on hydrolyzed spent coffee grounds (HSCG) for green antibacterial devices. J Clean Prod. 2020; 268: 122352. Available from: https://doi.org/10.1016/j.jclepro.2020.122352
Trongchuen K, Ounkaew A, Kasemsiri P, Hiziroglu S, Mongkolthanaruk W, Wannasutta R, et al. Bioactive Starch Foam Composite Enriched With Natural Antioxidants from Spent Coffee Ground and Essential Oil. Starch/Staerke. 2018; 70(7–8): 1–9.
Li CP, Weng MC, Huang SL. Preparation and characterization of pH sensitive chitosan/3-glycidyloxypropyl trimethoxysilane (GPTMS) hydrogels by Sol-Gel method. Polymers (Basel). 2020; 12(6): 1326.
Ardila N, Daigle F, Heuzey MC, Ajji A. Antibacterial activity of neat chitosan powder and flakes. Molecules. 2017; 22(1): 1–19.
Dhand V, Soumya L, Bharadwaj S, Chakra S, Bhatt D, Sreedhar B. Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater Sci Eng C. 2016;58:36–43. Available from: http://dx.doi.org/10.1016/j.msec.2015.08.018
Ebrahimi S, Farhadian N, Karimi M, Ebrahimi M. Enhanced bactericidal effect of ceftriaxone drug encapsulated in nanostructured lipid carrier against gram-negative Escherichia coli bacteria: Drug formulation, optimization, and cell culture study. Antimicrob Resist Infect Control. 2020; 9(1): 1–12.
Rochín-Medina JJ, Ramírez K, Rangel-Peraza JG, Bustos-Terrones YA. Increase of content and bioactivity of total phenolic compounds from spent coffee grounds through solid state fermentation by Bacillus clausii. J Food Sci Technol. 2018; 55(3): 915–923.
Ounkaew A, Kasemsiri P, Kamwilaisak K, Saengprachatanarug K, Mongkolthanaruk W, Souvanh M, et al. Polyvinyl Alcohol (PVA)/Starch Bioactive Packaging Film Enriched with Antioxidants from Spent Coffee Ground and Citric Acid. J Polym Environ. 2018; 26(9): 3762–3772. Available from: http://dx.doi.org/10.1007/s10924-018-1254-z
Liao C, Li Y, Tjong SC. Bactericidal and cytotoxic properties of silver nanoparticles. Int J Mol Sci. 2019; 20(2).
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275(1): 177–182.
Gholamali I, Asnaashariisfahani M, Alipour E. Silver Nanoparticles Incorporated in pH-Sensitive Nanocomposite Hydrogels Based on Carboxymethyl Chitosan-Poly (Vinyl Alcohol) for Use in a Drug Delivery System. Regen Eng Transl Med. 2019; 6: 138-153.
Badr AN, El‐attar MM, Ali HS, Elkhadragy MF, Yehia HM, Farouk A. Spent Coffee Grounds Valorization as Bioactive Phenolic Source Acquired Antifungal, Anti‐Mycotoxigenic, and Anti‐Cytotoxic Activities. Toxins. 2022; 14(2): 1–14.
Kim J, Lee CM. Wound healing potential of a polyvinyl alcohol-blended pectin hydrogel containing Hippophae rahmnoides L. extract in a rat model. Int J Biol Macromol. 2017; 99: 586–593. Available from: http://dx.doi.org/10.1016/j.ijbiomac.2017.03.014
Yadollahi M, Farhoudian S, Namazi H. One-pot synthesis of antibacterial chitosan/silver bio-nanocomposite hydrogel beads as drug delivery systems. Int J Biol Macromol. 2015; 79: 37–43. Available from: http://dx.doi.org/10.1016/j.ijbiomac.2015.04.032
Khorasani MT, Joorabloo A, Moghaddam A, Shamsi H, MansooriMoghadam Z. Incorporation of ZnO nanoparticles into heparinised polyvinyl alcohol/chitosan hydrogels for wound dressing application. Int J Biol Macromol. 2018; 114: 1203–1215. Available from: https://doi.org/10.1016/j.ijbiomac.2018.04.010
Kalantari K, Mostafavi E, Saleh B, Soltantabar P, Webster TJ. Chitosan/PVA hydrogels incorporated with green synthesized cerium oxide nanoparticles for wound healing applications. Eur Polym J. 2020; 134: 109853. Available from: https://doi.org/10.1016/j.eurpolymj.2020.109853
Zhang Y, Jiang M, Zhang Y, Cao Q, Wang X, Han Y, et al. Novel lignin–chitosan–PVA composite hydrogel for wound dressing. Mater Sci Eng C. 2019; 104: 110002. Available from: https://doi.org/10.1016/j.msec.2019.110002
Wang Y, Liu S, Yu W. Functionalized Graphene Oxide-Reinforced Chitosan Hydrogel as Biomimetic Dressing for Wound Healing. Macromol Biosci. 2021; 21(4): 1–10.
Yang J, Chen Y, Zhao L, Feng Z, Peng K, Wei A, et al. Preparation of a chitosan/carboxymethyl chitosan/AgNPs polyelectrolyte composite physical hydrogel with self-healing ability, antibacterial properties, and good biosafety simultaneously, and its application as a wound dressing. Compos Part B Eng. 2020; 197: 108139. Available from: https://doi.org/10.1016/j.compositesb.2020.108139
Luo C, Zhao Y, Sun X, Hu B. Developing high strength, antiseptic and swelling-resistant polyvinyl alcohol/chitosan hydrogels for tissue engineering material. Mater Lett. 2020; 280: 128499. Available from: https://doi.org/10.1016/j.matlet.2020.128499
Nie L, Deng Y, Li P, Hou R, Shavandi A, Yang S. Hydroxyethyl Chitosan-Reinforced Polyvinyl Alcohol/Biphasic Calcium Phosphate Hydrogels for Bone Regeneration. ACS Omega. 2020; 5(19): 10948–57.
Xiang X, Chen G, Chen K, Jiang X, Hou L. Facile preparation and characterization of super tough chitosan/poly(vinyl alcohol) hydrogel with low temperature resistance and anti-swelling property. Int J Biol Macromol. 2020; 142: 574–582. Available from: https://doi.org/10.1016/j.ijbiomac.2019.09.132
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