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The objectives of this study were to determine the essential oil composition and the anti-aging potential of essential oil and extracts from E. wandokthong rhizome. The essential oil was obtained by hydro-distillation of the fresh rhizome and its composition was identified by Gas Chromatography coupled to Mass Spectrometry system (GC-MS). Five major components, α-gurjunene (22.31%), alloaromadendrene (18.20%), 4-allyloxyimino-2-carene (5.83%), fenchyl acetate (5.44%), and γ-cadinene (5.19%), were identified in E. wandokthong essential oil. Moreover, the fresh rhizome and hydrodistillation residue were each extracted with ethanol, 50% ethanol-water, and water. The anti-aging potential of the essential oil and of the different extracts was evaluated for their total phenolic content, antioxidant, anti-tyrosinase, and keratinocyte proliferation promoting activities. The E. wandokthong rhizome extracts were about 5 times higher activity than that of the essential oil in all assays. The 50% ethanol-water extract provided the highest efficacy in all assays. Moreover, the antioxidant, anti-tyrosinase, and keratinocyte proliferation promoting activities of the hydrodistillation residue extracts were reduced about 2 times compared to those of the fresh rhizome. The results indicated that E. wandokthong rhizome essential oil and extracts have potential to use as fragrant and active ingredient in cosmetic products.
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