Bioethanol Production by Pichia stipitis Immobilized on Water Hyacinth and Thin-shell Silk Cocoon

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Published: Jul 27, 2022
DOI: https://doi.org/10.14416/j.asep.2021.03.006
Keywords:
Bioethanol production Cell immobilization Cell carrier Natural materials Pichia stipitis

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Suchata Kirdponpattara
Department of Chemical Engineering, Faculty Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand
Santi Chuetor
Department of Chemical Engineering, Faculty Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand
Malinee Sriariyanun
Department of Chemical and Process Engineering, Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand
Muenduen Phisalaphong
Department of Chemical Engineering, Faculty Engineering, Chulalongkorn University, Bangkok, Thailand

Abstract

Cell immobilization technique was applied in this study in order to examine effect of immobilized Pichia stipitis TISTR5806 on bioethanol production. Water hyacinth (WH) and thin-shell silk cocoon (CC) were used as cell carriers. Characteristics of the cell carriers were examined to explain the mechanism of bioethanol production. Carrier sizes and weights were optimized to improve bioethanol production. Moreover, stabilities of immobilized cells and carriers were evaluated. Because of high porosity, high surface area and good swelling ability of WH, cell immobilized on 1 g WH with 1 cm length produced the highest ethanol concentration at 13.3 g/L. Five cycles of a repeated batch of immobilized cell (IC) system on WH showed stable performance in ethanol production (8.2–10.4 g/L) with large numbers of the immobilized cells. The interaction between the immobilized cells and the WH surface were discovered.

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How to Cite
Kirdponpattara, S., Chuetor, S., Sriariyanun, M., & Phisalaphong, M. (2022). Bioethanol Production by Pichia stipitis Immobilized on Water Hyacinth and Thin-shell Silk Cocoon. Applied Science and Engineering Progress, 15(3), 4662. https://doi.org/10.14416/j.asep.2021.03.006
Issue
Vol. 15 No. 3 (2022)
Section
Research Articles

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