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The effect of [Emim][OAc] on Celluclast 1.5 L, Accellerase 1500, and IL-tolerant (MSL2) cellulase during the saccharification of carboxymethylcellulose (CMC), Avicel (AV), rice straw (RS) was studied in one pot process (pretreatment and saccharification). The inhibition caused by [Emim][OAc] (0.5–2.0 M) with substrate loading (20–50 mg/mL) were also evaluated. In most cases, the inhibition mode of saccharification for CMC and AV was identified to be uncompetitive inhibition when the concentration of [Emim][OAc] was higher than 0.5 M. Under the influence of 0.5 M of [Emim][OAc], the Critical Concentration of Substrate (CCS) values of the Celluclast 1.5 L and Accellerase 1500 on CMC hydrolysis were determined to be at 26.59 and 33.65 mg/mL, respectively. Also, increasing in [Emim][OAc] concentration could increase in CCS values, suggesting the positive effect of [Emim][OAc] on the improvement of enzymatic saccharification. This study provides insight into the process optimization for integration of [Emim][OAc] in one pot process of biorefinery.
P. Tantayotai, P. Pornwongthong, C. Muenmuang, T. Phusantisampan, and M. Sriariyanun, “Effect of cellulase-producing microbial consortium on biogas production from lignocellulosic biomass,” Energy Procedia, vol. 141, pp. 180–183, 2017.
M. Sriariyanun and K. Kitsubthawee, “Trends in lignocellulosic biorefinery for production of value-added biochemicals,” Applied Science and Engineering Progress, vol. 13, no. 2, pp. 283– 284, 2020, doi: 10.14416/j.asep.2020.02.005.
Y.-S. Cheng, P. Mutrakulcharoen, S. Chuetor, K. Cheenkachorn, P. Tantayotai, E. J. Panakkal, and M. Sriariyanun, “Recent situation and progress in biorefining process of lignocellulosic biomass: Toward green economy,” Applied Science and Engineering Progress, vol. 13, no. 4, pp. 299– 311, 2020, doi: 10.14416/j.asep.2020.08.002.
S. Zhang, W. C. Wang, F. X. Li, and J. Y. Yu, “Swelling and dissolution of cellulose in NaOH aqueous solvent systems,” Cellulose Chemistry and Technology, vol. 47, no. 9–10, pp. 671–679, 2013.
M. P. Gundupalli, H. Kajiura, T. Ishimizu, and D. Bhattacharyya, “Alkaline hydrolysis of coconut pith: process optimization, enzymatic saccharification, and nitrobenzene oxidation of Kraft lignin,” Biomass Conversion and Biorefinery, pp. 1–19, Jul. 2020, doi: 10.1007/ s13399-020-00890-z.
K. Karimi and M. J. Taherzadeh, “A critical review of analytical methods in pretreatment of lignocelluloses: Composition, imaging, and crystallinity,” Bioresource Technology, vol. 200, pp. 1008–1018, Jan. 2016.
M. P. Gundupalli and D. Bhattacharyya, “Sequential acid hydrolysis and enzymatic saccharification of coconut coir for recovering reducing sugar: Process evaluation and optimization,” Bioresource Technology Reports, vol. 6, pp. 70– 80, Jun. 2019.
K. Karimi and M. J. Taherzadeh, “A critical review on analysis in pretreatment of lignocelluloses: Degree of polymerization, adsorption/desorption, and accessibility,” Bioresource Technology, vol. 203, pp. 348–356, Mar. 2016.
T. Raj, R. Gaur, P. Dixit, R. P. Gupta, V. Kagdiyal, R. Kumar, and D. K. Tuli, “Ionic liquid pretreatment of biomass for sugars production: Driving factors with a plausible mechanism for higher enzymatic digestibility,” Carbohydrate Polymers, vol. 149, pp. 369–381, Sep. 2016.
P. Nargotra, V. Sharma, M. Gupta, S. Kour, and B. K. Bajaj, “Application of ionic liquid and alkali pretreatment for enhancing saccharification of sunflower stalk biomass for potential biofuelethanol production,” Bioresource Technology, vol. 267, pp. 560–568, Nov. 2018.
M. P. Gundupalli, K. Rattanaporn, S. Chuetor, W. Rodiahwati, and M. Sriariyanun, “Biocomposite production from Ionic liquids (IL) assisted processes using biodegradable biomass,” in Toward the Value-Added Biocomposites: Technology, Innovation and Opportunity. Florida: CRC Press, 2021.
P. Tantayotai, K. Rattanaporn, S. Tepaamorndech, K. Cheenkachorn, and M. Sriariyanun, “Analysis of an ionic liquid and salt tolerant microbial consortium which is useful for enhancement of enzymatic hydrolysis and biogas production,” Waste and Biomass Valorization, vol. 10, no. 6, pp. 1481–1491, Jun. 2019.
D. Stewart, “Lignin as a base material for materials applications: Chemistry, application and economics,” Industrial Crops and Products, vol. 27, no. 2, pp. 202–207, Mar. 2008.
N. I. Haykir and U. Bakir, “Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks,” Industrial Crops and Products, vol. 51, pp. 408–414, Nov. 2013.
B. Yang, X. Qin, H. Hu, C. Duan, Z. He, and Y. Ni, “Using ionic liquid (EmimAc)-water mixture in selective removal of hemicelluloses from a paper-grade bleached hardwood kraft pulp,” Cellulose, vol. 27, no. 16, pp. 9653–9661, Nov. 2020.
M. Sriariyanun, Q. Yan, I. Nowik, K. Cheenkachorn, T. Phusantisampan, and M. Modigell, “Efficient pretreatment of rice straw by combination of screw- press and ionic liquid to enhance enzymatic hydrolysis,” Kasetsart Journal - Natural Science, vol. 49, no. 1, pp. 146–154, 2015.
J. N. Pedersen, B. Pérez, and Z. Guo, “Stability of cellulase in ionic liquids: Correlations between enzyme activity and COSMO-RS descriptors,” Scientific Reports, vol. 9, no. 1, pp. 1–11, 2019.
D. Fu and G. Mazza, “Aqueous ionic liquid pretreatment of straw,” Bioresource Technology, vol. 102, no. 13, pp. 7008–7011, Jul. 2011.
P. Tantayotai, P. Rachmontree, W. Rodiahwati, K. Rattanaporn, and M. Sriariyanun, “Production of ionic liquid-tolerant cellulase produced by microbial consortium and its application in biofuel production,” Energy Procedia, vol. 100, pp. 155–159, Nov. 2016.
M. Sriariyanun, P. Tantayotai, P. Yasurin, P. Pornwongthong, and K. Cheenkachorn, “Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. isolated from rice paddy field soil,” Electronic Journal of Biotechnology, vol. 19, no. 1, pp. 23–28, Jan. 2016.
K. Ninomiya, A. Kohori, M. Tatsumi, K. Osawa, T. Endo, R. Kakuchi, C. Ogino, N. Shimizu, and K. Takahashi, “Ionic liquid/ultrasound pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid,” Bioresource Technology, vol. 176, pp. 169–174, Jan. 2015.
S. A. Campen, J. Lynn, S. J. Sibert, S. Srikrishnan, P. Phatale, T. Feldman, J. M. Guenther, J. Hiras, Y. T. A. Tran, S. W. Singer, P. D. Adams, K. L. Sale, B. A. Simmons, S. E. Baker, J. K. Magnuson, and J. M. Gladden, “Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger,” PLoS ONE, vol. 12, no. 12, p. e0189604, 2017.
M. Sriariyanun, P. Tantayotai, P. Yasurin, P. Pornwongthong, and K. Cheenkachorn, “Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. isolated from rice paddy field soil,” Electronic Journal of Biotechnology, vol. 19, pp. 23–28, Jan. 2016.
T. K. Ghose, “Measurement of cellulase activities,” Pure and Applied Chemistry, vol. 59, no. 2, pp. 257–268, 1987.
G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959.
K. Rattanaporn, P. Tantayotai, T. Phusantisampan, P. Pornwongthong, and M. Sriariyanun, “Organic acid pretreatment of oil palm trunk: Effect on enzymatic saccharification and ethanol production,” Bioprocess and Biosystems Engineering, vol. 41, no. 4, pp. 467–477, Apr. 2018.
K. Cheenkachorn, T. Douzou, S. Roddecha, P. Tantayotai, and M. Sriariyanun, “Enzymatic saccharification of rice straw under influence of recycled ionic liquid pretreatments,” Energy Procedia, vol. 100, pp. 160–165, Nov. 2016.
M. L. Shuler and F. Kargi, “Bioprocess engineering: Basic concepts,” Journal of Controlled Release, vol. 22, no. 3, p. 293, Nov. 1992.
J. I. Park, E. J. Steen, H. Burd, S. S. Evans, A. M. Redding-Johnson, T. Batth, P. I. Benke, P. D'haeseleer, N. Sun, K. L. Sale, J. D. Keasling, T. S. Lee, C. J. Petzold, A. Mukhopadhyay, S. W. Singer, B. A. Simmons, and J. M. Gladden, “A thermophilic ionic liquid-tolerant cellulase cocktail for the production of cellulosic biofuels,” PLoS ONE, vol. 7, no. 5, p. e37010, 2012.
H. Zhao, C. L. Jones, G. A. Baker, S. Xia, O. Olubajo, and V. N. Person, “Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis,” Journal of Biotechnology, vol. 139, no. 1, pp. 47–54, Jan. 2009.
M. Ouellet, S. Datta, D. C. Dibble, P. R. Tamrakar, P. I. Benke, C. Li, S. Singh, K. L. Sale, P. D. Adams, J. D. Keasling, B. A. Simmons, B. M. Holmes, and A. Mukhopadhyay, “Impact of ionic liquid pretreated plant biomass on Saccharomyces cerevisiae growth and biofuel production,” Green Chemistry, vol. 13, no. 10, p. 2743, 2011.
P. Engel, R. Mladenov, H. Wulfhorst, G. Jäger, and A. C. Spiess, “Point by point analysis: How ionic liquid affects the enzymatic hydrolysis of native and modified cellulose,” Green Chemistry, vol. 12, no. 11, pp. 1959–1966, 2010.
F. Hong, X. Guo, S. Zhang, S. Han, G. Yang, and L. J. Jönsson, “Bacterial cellulose production from cotton-based waste textiles: Enzymatic saccharification enhanced by ionic liquid pretreatment,” Bioresource Technology, vol. 104, pp. 503–508, Jan. 2012.
F. Islam and N. Roy, “Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses,” BMC Research Notes, vol. 11, no. 1, p. 445, Dec. 2018.
M. Abe, Y. Fukaya, and H. Ohno, “Extraction of polysaccharides from bran with phosphonate or phosphinate-derived ionic liquids under short mixing time and low temperature,” Green Chemistry, vol. 12, no. 7, pp. 1274–1280, 2010.
S. Vasheghani Farahani, Y. W. Kim, and C. A. Schall, “A coupled low temperature oxidative and ionic liquid pretreatment of lignocellulosic biomass,” Catalysis Today, vol. 269, pp. 2–8, 2016.
J. Shi, J. M. Gladden, N. Sathitsuksanoh, P. Kambam, L. Sandoval, D. Mitra, S. Zhang, A. George, S. W. Singer, B. A. Simmons, and S. Singh, “One-pot ionic liquid pretreatment and saccharification of switchgrass,” Green Chemistry, vol. 15, no. 9, pp. 2579–2589, 2013.
Y. Bi, C. Zhu, Z. Wang, H. Luo, R. Fu, X. Zhao, X. Zhao, and L. Jiang, “Purification and characterization of a glucose-tolerant β-glucosidase from black plum seed and its structural changes in ionic liquids,” Food Chemistry, vol. 274, pp. 422–428, 2019.
M. L. Carvalho, R. Sousa, U. F. Rodríguez- Zúñiga, C. A. G. Suarez, D. S. Rodrigues, R. C. Giordano, and R. L. C. Giordano , “Kinetic study of the enzymatic hydrolysis of sugarcane bagasse,” Brazilian Journal of Chemical Engineering, vol. 30, no. 3, pp. 437–447, 2013.
S. Shafique and S. Shafique, “Kinetic study of partially purified cellulase enzyme produced by Trichoderma viride FCBP-142 and its hyperactive mutants,” Mikrobiologiia, vol. 80, no. 3, pp. 356–365, 2011.
Z. Y. Wu, H. Zhang, Q. Q. Li, F. Q. Yang, and D. Q. Li, “Capillary electrophoresis-based online immobilized enzyme reactor for beta-glucosidase kinetics assays and inhibitors screening,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1110– 1111, pp. 67–73, 2019.
B. Pratto, R. B. A. de Souza, R. Sousa, and A. J. G. da Cruz, “Enzymatic hydrolysis of pretreated sugarcane straw: Kinetic study and semimechanistic modeling,” Applied Biochemistry and Biotechnology, vol. 178, no. 7, pp. 1430– 1444, 2016.
T. P. Silverstein, “When both Km and Vmax are altered, Is the enzyme inhibited or activated?,” Biochemistry and Molecular Biology Education, vol. 47, no. 4, pp. 446–449, Jul. 2019.
D. Bhattacharyya and K. S. Singh, “Colour removal and the effect of reactive dyes on growth substrate utilization by an unacclimated ethanolenriched anaerobic culture,” Water Quality Research Journal of Canada, vol. 45, no. 3, pp. 307–315, Aug. 2010.
Y. Hao, T. T. Li, X. L. Ma, and G. B. Guo, “Dissolution condition effect on cellulose in ionic liquid and the recycling of ionic liquid,” Applied Mechanics and Materials, vol. 644–650, pp. 5207–5210, 2014.
L. B. Johnson, S. Park, L. P. Gintner, and C. D. Snow, “Characterization of supercharged cellulase activity and stability in ionic liquids,” Journal of Molecular Catalysis B: Enzymatic, vol. 132, pp. 84–90, 2016.
W. Li, L. Wang, R. Zhou, and Y. Mu, “Ionic liquid induced inactivation of cellobiohydrolase I from Trichoderma reesei,” Green Chemistry, vol. 17, no. 3, pp. 1618–1625, 2015.
M. Paulraj Gundupalli, A. Sahithi S T, Y.-S. Cheng, P. Tantayotai, and M. Sriariyanun, “Differential effects of inorganic salts on cellulase kinetics in enzymatic saccharification of cellulose and lignocellulosic biomass,” Bioprocess and Biosystems Engineering, Jun. 2021, doi: 10.1007/s00449-021-02607-6.