DNA Binding Activity of Marine Shrimp LvProfilin

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Published: Jul 27, 2022
DOI: https://doi.org/10.14416/j.asep.2021.10.002
Keywords:
Profilin Marine shrimp Phylogenetic tree Tissue distribution SWISS-MODEL DNA binding protein

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Yanisa Laoong-u-thai
Department of Chemical Engineering, Faculty of Engineering, Burapha University, Chonburi, Thailand Bioengineering Program, Faculty of Engineering, Burapha University, Chon Buri, Thailand
Warapond Wanna
Center for Genomics and Bioinformatics Research, Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla, Thailand
Autaipohn Kaikaew
College of Research Methodology and Cognitive Science, Burapha University, Chon Buri, Thailand

Abstract

Shrimp farming is an important business in Thailand and worldwide. The study of molecular biology and biochemical pathway of the key molecules controlling muscle growth is essential to improve shrimp livestock. Profilin is a pivotal protein in muscle formation, especially actin protein. Its nuclear function has been reported in many species for gene regulation. Here in this work, we characterized the function of LvProfilin, marine shrimp profilin from Litopenaeus vannamei, both in silico and in vitro. The phylogenetic tree of LvProfilin among organisms and its 3D protein structure showed that LvProfilin was highly conserved among shrimp and arthropods. The homology modeling of its 3D structure revealed three alpha-helices and six beta-strands similar to most eukaryotic profilins. To interpret its possible function, the gene expression of LvProfilin in various tissues was performed. We found that this gene was expressed in various tissues. This result may imply that LvProfilin could share a common function in all tissues. Nuclear activity has been a promising function of LvProfilin. We performed a DNA/RNA binding prediction analysis using DRNApred. The result indicated that Lysine-90 and Threonine-91 were the putative DNA-binding sites with the probability of 63.12% and 54.16%, respectively. Its binding activity was confirmed in vitro, which bound stronger to single-strand DNA than double-strand DNA. To our best knowledge, this is the first report of the DNA binding activity of profilin in invertebrates.

Article Details

How to Cite
Laoong-u-thai, Y. ., Wanna, W., & Kaikaew, A. (2022). DNA Binding Activity of Marine Shrimp LvProfilin. Applied Science and Engineering Progress, 15(3), 5522. https://doi.org/10.14416/j.asep.2021.10.002
Issue
Vol. 15 No. 3 (2022)
Section
Research Articles

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