Advantages of Electro-deposited Gold on Carbon Electrodes for NT-proBNP Immunosensor for Development of Heart Failure Test Kit

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Pongsakorn Aiemderm
Kanchana Monkhang
Sureeporn Wongjard
Kiattawee Choowongkomon
Napachanok Mongkoldhumrongkul Swainson Mongkoldhumrongkul Swainson
Chaiya Prasittichai
Charoenkwan Kraiya

Abstract

Accurate measurement of the N-terminal pro B-type natriuretic peptide (NT-proBNP) in serum is important for the diagnosis of heart failure (HF). Carbon screen-printed electrodes (SPCEs) modified with graphene oxide (GO) or gold (Au) were compared for the construction of NT-proBNP immunosensors. NT-proBNP and its recognition unit, a single-chain variable fragment fused with alkaline phosphatase (scFv-AP), were expressed and purified. The currents of the electrodes immobilized with scFv-AP were measured after adding an ethanolamine (ETA), blank and NT-proBNP in either phosphate buffer saline (PBS) or human serum. SPCE/Au had lower mean baseline slopes than for SPCE/GO for all measurements, in both PBS and serum, indicating greater accuracy for SPCE/Au. None of the measurements in PBS had statistically different peak currents between SPCE/GO and SPCE/Au; however, there was a significant difference with the serum. The significant reduction of SPCE/ GO peak currents after applying serum blank implied non-specific absorption on the surface. The peak current of 300 pg/mL of NT-proBNP in the serum measured on SPCE/Au was significantly higher (by a factor of three) than on SPCE/GO, suggesting the possibility of using SPCE/Au to detect NT-proBNP at higher concentrations. The binding efficiency of scFv-AP to NT-proBNP did not depend on the electrodes, as shown by the similar delta peak-currents (Blank-Target). Thus, immobilized scFv-AP on SPCE/Au electrodes had good potential to accurately detect NT-proBNP in serum, for use in the fabrication of an HF test kit.

Article Details

How to Cite
Aiemderm, P., Monkhang, K., Wongjard, S., Choowongkomon, K., Mongkoldhumrongkul Swainson, N. M. S., Prasittichai, C., & Kraiya, C. (2024). Advantages of Electro-deposited Gold on Carbon Electrodes for NT-proBNP Immunosensor for Development of Heart Failure Test Kit. Applied Science and Engineering Progress, 17(2), 7004. https://doi.org/10.14416/j.asep.2023.10.004
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Research Articles

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