Fish Protein Hydrolysate Production by Acid and Enzymatic Hydrolysis

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Nicharee Wisuthiphaet
Sopa Klinchan
Sasithorn Kongruang


The objective of this study was to optimize the condition for fish protein hydrolysate production from minced by-catch fish by using hydrochloric acid and 2 protease enzymes; Papain and Alcalase. Taguchi design and Central Composite Design (CCD) were applied for experimental design to evaluate the degree of hydrolysis. Response surface methodology was performed in order to determine the optimal production conditions. The optimal condition for acid hydrolysis was 4 mol/L of hydrochloric acid at 100oC for 90 minutes which yielded 50.70% degree of hydrolysis. For enzymatic hydrolysis, Alcalase is the most suitable protease enzyme for fish protein hydrolysate production. The optimal condition was 6 % (w/w) Alcalase concentration at the temperature of 61.23 oC and the reaction time of 27.36 minutes resulting in 88.9% of degree of hydrolysis. Amino acid profiles of fish protein hydrolysates hydrolyzed under the optimal condition were analyzed by HPLC and the results showed that fish protein hydrolyzed by Papain had highest nutritional properties. Glutamic acid had the highest percentage (16.35%) followed by Aspartic acid (10.41%) and Lysine (8.48%).

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How to Cite
Wisuthiphaet, N., Klinchan, S., & Kongruang, S. (2016). Fish Protein Hydrolysate Production by Acid and Enzymatic Hydrolysis. Applied Science and Engineering Progress, 9(4). Retrieved from
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Author Biography

Nicharee Wisuthiphaet, <p>Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand</p><p>Bioprocess Engineering and Biotechnology Research Center, STRI, KMUTNB, Bangkok, Thailand</p>

Department of Biotechnology, Faculty of Applied Science, KMUTNB