Phytochemical contents and antioxidant activities of Thai herbal tea from leaves of Morus alba and Citrus hystrix

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Rachanee Nammatra

Abstract

A herbal tea composed of Morus alba L. (leaves): Citrus hystrix DC. (leaves); 1: 1 (w:w) was extracted using aqueous media. Phytochemicals screening determined total phenolic content (TPC), total flavonoid content (TFC), and the identification of flavonoids and phenolic acids of the extract using High-Performance Liquid Chromatography (HPLC). Antioxidant activities, including 2,2-diphenyl-1-picrylhydrazy (DPPH) and ferric reducing antioxidant power (FRAP) were also assayed.  The results showed that the TPC and TFC of the extract were 8.18 ± 0.26 mg GE/g Ext and 6.79 ± 0.75 mg RE/g Ext, respectively. The HPLC analyses of flavonoid and phenolic compounds indicated the presence of catechin, myricetin, quercetin, kaempferol, gallic acid, syringic acid, and coumaric acid as bioactive compounds. Antioxidant activities for DPPH and FRAP were 3.51 ± 0.03 mg TE/g Ext and 15.64 ± 0.36 mM Fe2+ E/g Ext, respectively. This finding indicates that Thai herbal tea derived from leaves of M. alba and C. hystrix has an inhibitory free radical effect and has the potential to promote health following herbal tea consumption.

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How to Cite
Nammatra, R. (2021). Phytochemical contents and antioxidant activities of Thai herbal tea from leaves of Morus alba and Citrus hystrix. ournal of cience and gricultural echnology, 2(1), 21-27. https://doi.org/10.14456/jsat.2021.4
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Research Article

References

Abirami, A., Nagarani, G. and Siddhuraju, P. 2015. Hepatoprotective effect of leaf extracts from Citrus hystrix and C. maxima against paracetamol induced liver injury in rats. Food Sci Hum Well. 4(1): 35-41.

Ali, M., Akhter, R., Narjish, S.N., Shahriar, M. and Bhuiyan, M.A. 2015. Studies of preliminary phytochemical screening, membrane stabilizing activity, thrombolytic activity and in-vitro antioxidant activity of leaf extract of Citrus hystrix. IJPSR: 6(6): 2367-2374.

Benzie, I.F.F. and Strain, J.J. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power": The FRAP Assay. Anal. Biochem. 239(1): 70-76.

Borneo, R., Leon, A.E., Aguirre, A., Ribotta, P. and Cantero, J.J. 2009. Antioxidant capacity of medicinal plants from the Province of Cordoba (Argentina) and their in vitro testing in a model food system. Food Chem. 112: 664–670.

Butkhup, L. and Samappito, S. 2008. Analysis of anthocyanin, flavonoids and phenolic acids in tropical bignay berries. Int. J. Fruit Sci. 8: 15–34.

Butryee, C., Sunbgpuang, P. and Chitchumroonchokchai, C. 2009. Effect of processing on the flavonoid content and antioxidant capacity of Citrus hystrix leaf. Int J Food Sci Nutr. 60(S2): 162-174.

Chan, E.W.C. and Lim, Y.Y. 2006. Antioxidant activity of Thunbergia laurifolia tea. J. Trop. For. Sci. 18(2): 130–136.

Chan, E.W.C., Lye, P.Y., Tan, L.N., Eng, S.Y., Tan, Y.P. and Wong, Z.C. 2012. Effects of drying method and particle size on the antioxidant properties of leaves and teas of Morus alba, Lagerstroemia speciosa and Thunbergia laurifolia. Chem. Ind. Chem. Eng. Q. 18(3): 465−472.

Chan, E.W.C., Lye P.Y., and Wong, S.K. 2016. Phytochemistry, pharmacology, and clinical trials of Morus alba. CJNM. 14(1): 0017-0030.

Choi, E.M. and Hwang, J.K. 2005. Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages. Fitoterapia. 76(7-8): 608-613.

Do, Q.D., Angkawijaya, A.E., Tran‑Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S. and Ju, Y-H. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 22(3): 296-302.

Duthie, G. and Crozier, A. 2000. Plant-derived phenolic antioxidants. Curr. Opin. Lipidol. 11(1): 43-47.

Hansawasdi, C. and Kawabata, J. 2006. α-Glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2. Fitoterapia. 77(7-8): 568-573.

Hwang, S.H., Li, H.M., Lim, S.S., Wang, Z., Hong, J. and Huang, B. 2016. Evaluation of a standardized extract from Morus alba against α-glucosidase inhibitory effect and postprandial antihyperglycemic in patients with impaired glucose tolerance: A randomized double-blind clinical trial. Evid Based Complement Alternat Med. 2016: https://doi.org/10.1155/2016/8983232.

Iqbal, E., Salim, K.A. and Lim, L.B.L. 2015. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J. King Saud Univ. Sci. 27: 224–232.

Jorjong, S., Butkhup, L. and Samappito, S. 2015. Phytochemicals and antioxidant capacities of Mao-Luang (Antidesma bunius L.) cultivars from Northeastern Thailand. Food Chem. 181: 248-255.

Laohavechvanich, P., Muangnoi, C., Butryee, C. and Kriengsinyos, W. 2010. Protective effect of makrut lime leaf (Citrus hystrix) in HepG2 cells: Implications for oxidative stress. Sci Asia. 36: 112–117.

Lawrence, B.W., Hogg, J.W., Terhune, S.J. and Podimuang, V. 1971. Constituents of the leaf and peel oils of Citrus hydrix DC. Phytochemistry. 10: 1404–1405.

Mahmoud, M.A., El‑Twab, S.M.A. and Abdel‑Reheim, E.S. 2017. Consumption of polyphenol‑rich Morus alba leaves extract attenuates early diabetic retinopathy: the underlying mechanism. Eur. J. Nutr. 56: 1671–1684.

Mojulat, M.B.C. and Surugau, N. 2018. Effect of extraction conditions of Carica papaya leaves aqueous extracts and its resulting infusion with “kelulut” honey to its antioxidant activity. ASM Sc. J. 11(2): 75-86.

Nammatra, R., Srihawong, T. and Chaloemram, C. 2021. Evaluation of phytochemical constituents and antioxidant activities of different formula of heart tonic herbal teas. JSSM. 16(2): 94-104.

Radojković, M.M., Zeković, Z.P., Vidović, S.S., Kočar, D.D. and Mašković, P.Z. 2012. Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts. Hem. ind. 66(4): 547–552.

Ratseewo, J., Tangkhawanit, E., Meeso, N., Kaewseejan, N. and Siriamornpun, S. 2016. Changes in antioxidant properties and volatile compounds of kaffir lime leaf as affected by cooking processes. Int. Food Res. J. 23(1): 188-196.

Ravikumar, C. 2014. Review on Herbal Teas. J. Pharm. Sci. & Res. 6(5): 236-238.

Saeed, N., Khan, M.R. and Shabbir, M. 2012. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Compl Alternative Med. 12(No.221): 1-12.

Sharma, V. and Agarwal, A. 2015. Physicochemical and antioxidant assays of methanol and hydromethanol extract of ariel parts of Indigofera tinctoria Linn. Indian J. Pharm. Sci. 77(6): 729–734.

Singleton, V.L., Orthofer, R. and Lamuela‑ Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin‑ ciocalteu reagent. Methods Enzymol. 299: 152-178.

Soonthornsit, N., Pitaksutheepong, C., Hemstapat, W., Utaisincharoen, P. and Pitaksuteepong, T. 2017. In vitro anti-inflammatory activity of Morus alba L. stem extract in LPS-stimulated RAW 264.7 cells. Evid Based Complement Alternat Med. 2017: https://doi.org/10.1155/2017/3928956.

Thabti, I., Elfalleh, W., Hannachi, H., Ferchichi, A. and Campos, M.D.G. 2012. Identification and quantification of phenolic acids and flavonol glycosides in Tunisian Morus species by HPLC-DAD and HPLC–MS. J. Funct. Foods. 4: 367–374.

Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L. and Byrne, D.H. 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 19: 669-675.

Tunjung, W.A.S., Cinatl, J., Michaelis, M. and Smales, C.M. 2015. Anti-cancer effect of Kaffir Lime (Citrus hystrix DC) leaf extract in cervical cancer and neuroblastoma cell lines. Procedia Chem. 14: 465-468.

Wijaya, Y.A., Widyadinata, D., Irawaty, W. and Ayucitra, A. 2017. Fractionation of phenolic and flavonoid compounds from kaffir lime (Citrus hystrix) peel extract and evaluation of antioxidant activity. Reaktor. 17(3): 111-117.

Yadav, A.V., Kawale, L.A. and Nade, V.S. 2008. Effect of Morus alba L. (mulberry) leaves on anxiety in mice. Indian J. Pharmacol. 40(1): 32–36.