The effects of energy and protein content in maize forage-based complete diet on in vitro ruminal fermentation, gas production, and feed degradability

Main Article Content

Mirwan Budianto


This research aimed to evaluate the effects of energy and protein contents in a complete diet on in vitro gas production, rumen fermentation, and feed degradability. The experiment used a 3x3 factorial arrangement in
a randomized block design with two factors and three replications. The first factor was energy content in
the complete diets; E1=12.5MJ/kg DM, E2=13.5MJ/kg DM, and E3=14.5 MJ/kg DM. The second factor was protein content in the complete diets; P1=10.5%, P2=13.5%, and P3=16.5%. The complete diet was composed of maize forage silage 37.5 % w/w DM, elephant grass (Pennisetum purpureum) 12.5 % w/w DM, and concentrate 50.0 % w/w DM. The concentrate was composed of commercial dairy concentrate feed produced by SAE local dairy cooperative, cassava waste, soybean meal, rice bran, and coffee husk. All of the treatment diets were tested using in vitro gas production test. The variables were total, potential, and rate of in vitro gas production, NH3 concentration, efficiency of microbial protein synthesis (EMPS), dry matter degradability (DMD), and organic matter degradability (OMD). Either energy or protein content of the treatment diets had a highly significant effect (P<0.01) on the total, potential, and rate of in vitro gas production, NH3 concentration, EMPS, DMD, and OMD, but not for the treatment combination. An increase in energy and protein content in the treatment diets increased
the value of all parameters but decreased EMPS.

Article Details

How to Cite
Budianto, M. (2023). The effects of energy and protein content in maize forage-based complete diet on in vitro ruminal fermentation, gas production, and feed degradability. Journal of Science and Agricultural Technology, 4(1), 27–33.
Research Article


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