Isolation, identification and antimicrobial activities of actinobacteria associated with fire ant, Solenopsis geminata
Keywords:
Actinobacteria, Fire ant, Solenopsis geminata, Antimicrobial activities, DiversityAbstract
Actinobacteria, Gram-stain positive with high G+C content bacteria, have been well known as the antibiotic producer. To find out the new habitat for searching new actinobacteria, we tried to isolate the actinobacteria from the fire ant, Solenopsis geminata, collected in Thailand. Totally, 6 actinobacteria were isolated and were identified, using 16S rRNA gene, as Streptomyces (2 isolates) and Nocardia (4 isolates) species. Both Streptomyces isolates showed antimicrobial activities against tested microorganism used in this study. Moreover, Nocardia isolate SE2 showed low 16S rRNA gene similarity to those known actinobacterial species and represented the candidate of novel actinobacterial species.
References
Bai L, Liu c, Guo L, Piao C, Li Z, Li J et al. (2016) Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicas Mayr. Antonie Van Leeu 109:253-261.
Beemelmanns C, Guo H, Rischer M, Poulsen M (2016) Natural products from microbes associated with insect. BEILSTEIN J Org Chem 12:314-317
Beemelmanns C, Ramadhar TR, Kim KH, Kalssen JL, Cao S, Wyche TP et al. (2017) Macrotermycins A-D, glycosy-lated macrolactams from a termite-associated Amycolatopsis sp. M39. Org Lett 19:1000-1003
Carr G, Derbyshire ER, Caldera E, Currie CR Clardy J (2012a) Antibiotic and antimalarial quinones from fungus-growing ant-associated Pseudono-cardia sp. J Nat Prod 75:1806-1809
Carr G, Pousen M, Klassen JL, You Y, Wyche TP, Bugni TS et al. (2012b) Microtermolides A and B from termite-associated Streptomyces sp. and structural revision of vinylamycin. Org Lett 14:2822-2825
Currie CR, Scott JA, Summerbell RC, Malloch D (1999) Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature 398:701-704
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368-376
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791
Guo ZK, Liu SB, Jiao RH, Wang T, Tan X, Ge HM (2012) Angucyclines from an insect-derived actinobacterium Amycolatopsis sp. HCa1 and their cytotoxic activity Bioor Med Chem Lett 22:7490-7493
Han C, Liu C, Zhao J, Guo L, Lu C, Li J et al. (2016) Microbispora camponoti sp. nov., a novel actinomycete isolated from the cutical of Camponotus japonicas Mayr. Antonie Van Leeu 109:215-223
Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501-509
Ishak HD, Plowes R, Sen R, Kellner K, Meyer E, Estrada DA et al. (2011) Bacterial diversity in Solenopsis invicta and Solenopsis geminate and colonies characterized by 16S amplicon 454 pyrosequencing. Microb Evol 61:821831
Iwai Y, Kora A, Takahashi Y, Kayashi Y, Awaya J, Masuma R et al (1987) Production of deoxyfrenolicin and a new antibiotic, frenolicin B by Streptomyces roseofulvus strain AM-3867. J Antibiot 31:959-965
Kageyama A, Poonwan N, Yazawa K, Suzuki S, Kroppenstedt RM, Mikami Y (2004) Nocardia vermiculata sp. nov. and Nocardia thailandica sp. nov. isolated from clinical specimens. Actinomycetologica 18:27-33
Kang HR, Lee D, Benndorf R, Jung WH, Beemelmanns C, Kang KS et al. (2016) Termisoflavones A-C, isoflavonoid glycosides from termite-associated Sterptomyces sp. RB81. J Nat Prod 79:3072-3078
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for biffer datasets. Mol Biol Evol 33:1870-1874
Lane DJ (1994) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic Acid Techniques in Bacterial Systematics. Wiley, Chichester, pp 115-175
Liu C, Bai L, Ye L, Zhao J, Yan K, Xiang W et al. (2016) Nocardia lasii sp. nov., a novel actinomycete isolated from the cuticle of an ant (Lasius fuliginosus L). Int J Syst Evol Microbiol 109:1513-1520
Matsumoto A, Takahashi Y (2017) Endophytic actinomycetes: promising source of novel bioactive compounds. J Antibiot 70:517-519
Oh D-C, Poulsen M, Currie CR, Clardy J (2011) Sceliphrolactam, a polyene macrocyclic lactam from a wasp-associated Streptomyces sp. Org Lett 13:752-755
Qin Z, Munnoch JT, Devine R, Holmes NA, Seipke RF, Wilkonson KA et al. (2017) Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants. Chem Sci 8:3218
Ruimy r, Riegle P, Carlotti A, Boiron P, Bernardin G, Montel H et al. (1996) Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases. Int J Syst Bacteriol 46:259-264
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313-340
Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold stands. Microbiol Today 33:152-155
Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W, Kirtikara K et al. (2009) Planotetra-spora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992-997
Tamaoka J (1944) Determination of DNA base composition. In: Goodfellow M, O’Donnel AG(eds) Chemical Methods in Prokaryotic Systematics. Wiley, Chichester, pp 463-470
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882
Xiang W, Yu C, Liu C, Zhao J, Yang L, Xie B (2014) Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vinica Roger). Int J Syst Evol Microbiol 64:495-500
Ylihonko K, Tuikkanen J, Jusssila S, Cong L, Mantsala P (1996) A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalator: sequence analysis and complemen-tation of early-block mutations in the anthracycline pathway. Mol Gen Genet 251:113-120
Yoon SH, Ha SM, Kown S, Lim J, Kim Y et al. (2017) Introducing EzBio-Cloud: a taxonomically united database of 16S rRNA gene sequence and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613-1617
Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R et al. (2013) Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger). Antonie Van Leeu 104:1013-1019
Beemelmanns C, Guo H, Rischer M, Poulsen M (2016) Natural products from microbes associated with insect. BEILSTEIN J Org Chem 12:314-317
Beemelmanns C, Ramadhar TR, Kim KH, Kalssen JL, Cao S, Wyche TP et al. (2017) Macrotermycins A-D, glycosy-lated macrolactams from a termite-associated Amycolatopsis sp. M39. Org Lett 19:1000-1003
Carr G, Derbyshire ER, Caldera E, Currie CR Clardy J (2012a) Antibiotic and antimalarial quinones from fungus-growing ant-associated Pseudono-cardia sp. J Nat Prod 75:1806-1809
Carr G, Pousen M, Klassen JL, You Y, Wyche TP, Bugni TS et al. (2012b) Microtermolides A and B from termite-associated Streptomyces sp. and structural revision of vinylamycin. Org Lett 14:2822-2825
Currie CR, Scott JA, Summerbell RC, Malloch D (1999) Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature 398:701-704
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368-376
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791
Guo ZK, Liu SB, Jiao RH, Wang T, Tan X, Ge HM (2012) Angucyclines from an insect-derived actinobacterium Amycolatopsis sp. HCa1 and their cytotoxic activity Bioor Med Chem Lett 22:7490-7493
Han C, Liu C, Zhao J, Guo L, Lu C, Li J et al. (2016) Microbispora camponoti sp. nov., a novel actinomycete isolated from the cutical of Camponotus japonicas Mayr. Antonie Van Leeu 109:215-223
Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501-509
Ishak HD, Plowes R, Sen R, Kellner K, Meyer E, Estrada DA et al. (2011) Bacterial diversity in Solenopsis invicta and Solenopsis geminate and colonies characterized by 16S amplicon 454 pyrosequencing. Microb Evol 61:821831
Iwai Y, Kora A, Takahashi Y, Kayashi Y, Awaya J, Masuma R et al (1987) Production of deoxyfrenolicin and a new antibiotic, frenolicin B by Streptomyces roseofulvus strain AM-3867. J Antibiot 31:959-965
Kageyama A, Poonwan N, Yazawa K, Suzuki S, Kroppenstedt RM, Mikami Y (2004) Nocardia vermiculata sp. nov. and Nocardia thailandica sp. nov. isolated from clinical specimens. Actinomycetologica 18:27-33
Kang HR, Lee D, Benndorf R, Jung WH, Beemelmanns C, Kang KS et al. (2016) Termisoflavones A-C, isoflavonoid glycosides from termite-associated Sterptomyces sp. RB81. J Nat Prod 79:3072-3078
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for biffer datasets. Mol Biol Evol 33:1870-1874
Lane DJ (1994) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic Acid Techniques in Bacterial Systematics. Wiley, Chichester, pp 115-175
Liu C, Bai L, Ye L, Zhao J, Yan K, Xiang W et al. (2016) Nocardia lasii sp. nov., a novel actinomycete isolated from the cuticle of an ant (Lasius fuliginosus L). Int J Syst Evol Microbiol 109:1513-1520
Matsumoto A, Takahashi Y (2017) Endophytic actinomycetes: promising source of novel bioactive compounds. J Antibiot 70:517-519
Oh D-C, Poulsen M, Currie CR, Clardy J (2011) Sceliphrolactam, a polyene macrocyclic lactam from a wasp-associated Streptomyces sp. Org Lett 13:752-755
Qin Z, Munnoch JT, Devine R, Holmes NA, Seipke RF, Wilkonson KA et al. (2017) Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants. Chem Sci 8:3218
Ruimy r, Riegle P, Carlotti A, Boiron P, Bernardin G, Montel H et al. (1996) Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases. Int J Syst Bacteriol 46:259-264
Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313-340
Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold stands. Microbiol Today 33:152-155
Suriyachadkun C, Chunhametha S, Thawai C, Tamura T, Potacharoen W, Kirtikara K et al. (2009) Planotetra-spora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992-997
Tamaoka J (1944) Determination of DNA base composition. In: Goodfellow M, O’Donnel AG(eds) Chemical Methods in Prokaryotic Systematics. Wiley, Chichester, pp 463-470
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876-4882
Xiang W, Yu C, Liu C, Zhao J, Yang L, Xie B (2014) Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vinica Roger). Int J Syst Evol Microbiol 64:495-500
Ylihonko K, Tuikkanen J, Jusssila S, Cong L, Mantsala P (1996) A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalator: sequence analysis and complemen-tation of early-block mutations in the anthracycline pathway. Mol Gen Genet 251:113-120
Yoon SH, Ha SM, Kown S, Lim J, Kim Y et al. (2017) Introducing EzBio-Cloud: a taxonomically united database of 16S rRNA gene sequence and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613-1617
Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R et al. (2013) Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger). Antonie Van Leeu 104:1013-1019
Downloads
Published
2018-04-30
Issue
Section
Original Articles
License
Copyright Notice: a copyright on any article in the published journal is retained by the Ramkhamhaeng International Journal of Science and Technology. Readers or Users grant the right to use of the Article contained in the Content in accordance with the Creative Commons CC BY-NC-ND license and the Data contained in the Content in accordance with the Creative Commons CC BY-NC-ND.
