Screening and optimization of oleaginous marine fungi for lipid production as a possible source for biodiesel

Abstract

Biodiesel is a good alternative energy source, but the plant oils and animal fats use feedstock it can apply in cooking as a result of to increase in the cost of food products. The present research finds that single-cell oils (SCOs) or oil from microorganisms produce triglycerides similar to plant oils. This research aims to screen and find appropriate culture media conditions for oleaginous marine fungi. In the experiment, the marine fungi were isolated from marine organisms were collected from Mu Koh Yao and Mu Koh Samaesarn using the tissue transplanting method and screened by Sudan Black B staining and extracting lipid from fungi using chloroform with methanol. Studies for five parameters namely, glucose concentration, peptone concentration, incubation period, pH, and water type for optimum conditions. The result of fungi isolated from both sites found fifty-five isolates. The lipid content in the marine fungal, Aspergillus sp. MMERU 24 isolated from sponge (Amphimedon sp.) at Mu Koh Yao showed lipid content at 20.90±1.71% per dried biomass. Glucose concentration, peptone concentration, incubation period, pH, and water type of 30 g/L, 1 g/L, 8 days, 5, and distilled water 100% produced the highest lipid content at 25.47%, 23.02%, 26.67%, 19.23%, and 20.1% respectively, were the optimum culture media condition of Aspergillus sp. MMERU 24. From these experiments, be able to develop fungal to biodiesel production in the further, which is environmentally friendly and has fewer emissions compared to other fuels, and following the policy toward net-zero emissions.