Evaluation of Antifungal activity of Piper genus Inhibition Pyricularia oryzae Causing Rice Blast Disease
Keywords:
Piper, Antifungal, Rice blast, Pyricularia oryzae, InhibitionAbstract
This research was finding out that bioactivities of piper genus extracts were inhibiting growth of Pyricularia oryzae cause of rice blast disease. The crude extract of four sources such as Piper retrofractum Vahl, Piper betle L., Piper sarmentosum Roxb., and Piper nigrum L., were screened for their efficacy antifungal activity under in vitro method. The percolation techniques used solvents hexane, ethyl acetate and methanol respectively for extraction, and the samples were tested in vitro at 10, 30 and 50 mg/ml concentration by the poisoned food technique. Results, phytochemicals screening in the sample extracts found alkaloids, flavonoids, phenols, steroids, and terpenoids. The efficacies of plant extracts inhibit mycelial growth of P.oryzae increase according to concentration. The E of P. betle L. had the highest effectiveness inhibition fungicide that wholly (100%) inhibited the fungus. The tested extracts had antifungal effect with the high inhibition percent (>90%) such as H of P. betle L. P. sarmentosum Roxb., and P. nigrum L.; E of P. retrofractum Vahl., Piper betle L., P. sarmentosum Roxb., and M of P. betle L. Conclude the piper genus extracts could be used as inhibiting choice to mycelial growth of P. oryzae and a source for development of organic fungicides; in agricultural safety.
References
Yin C, Zhu Y, Li X, Y Lin. (2021) Molecular and genetic aspects of grain number determination in rice (Oryza sativa L.). Int J Sci. 22:728.
Tripathi KK, Govila OP, Warrier R, Ahuja V. (2011) Biology of Oryza sativa L. (rice). Series of Crop Specific Biology Documents.
Kiguchi M, Takata K, Hanasaki N, Archevarahuprok B, Champathong A, Ikoma E, Jaikaeo C, Kaewrueng S, Kanae S, Kazama S, Kuraji K, Matsumoto K, Nakamura S, Nguyen-Le D, Noda K, Piamsa-Nga N, Raksapatcharawong M, Rangsiwanichpong P, Ritphring S, Shirakawa H, Somphong C, Srisutham M, Suanburi D, Suanpaga W, Tebakari T, Trisurat Y, Udo K, Wongsa S, Yamada T, Yoshida K, Kiatiwat T, Oki T. (2021) A review of climate-change impact and adaptation studies for the water sector in Thailand. Environ Res Lett. 16:1-35.
Longya A, Talumphai S, Jantasuriyarat C. (2020) Morphological characterization and genetic diversity of rice blast fungus, Pyricularia oryzae, from Thailand using ISSR and SRAP markers. J Funji. 6:38; DOI:10.3390/jof6010038.
Persaud R, Khan A, Isaac WA, Ganpat W, Saravanakumar D. (2019) Plant extracts, bio agents and new generation fungicides in the control of rice sheath blight in Guyana. Crop Prot. 119:30-37.
Agbowuro GO, Afolabi MS, Olamiriki EF, Awoyemi SO. (2020) Rice blast disease (Magnaporthe oryzae): a menace to rice production and humanity. Int J Path Res. 4(3):32-39.
Flora G, Rani SMA. (2012) An approach towards control of blast by foliar application of seaweed concentrate. Sci Res Rep. 2:213-217.
Panwey S. (2018) Effect of botanicals for management of leaf blast and enhancing yield traits in rice. Curr Bot. 9:17-21.
Suprapta DN. (2014) Pestisida Nabati Potensidan Prospek Pengembangan. Pelawa Sari. 31-45.
Kassankogno AI, Ouedraogo I, Tiendrebeogo A, Ouedraogo L, Sankara P. (2015) In vitro evaluation of the effect of aqueous extracts of Agave sisalana and Cymbopogon citratus on mycelial growth and conidia production of Pyricularia oryzae, causal agent of rice blast. J Appl Biosci.89:8272-8280.
Suriani NL, Suprapta DN, Sudana IM, Temaja IGRM. (2015) Antifungal activity of Piper caninum against Pyricularia oryzae Cav. the cause of rice blast disease on rice. J Bio Agri Heal. 5(8):72-78.
Suriani NL. (2018) Bioactive substance use of leaf extract of Piper Caninum Blume pressing for blast disease and increase production in rice. Int J Life Sci. 2(2):42-50.
Rao USM, Abdurrazak M, Mohd KS. (2016) Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of Musa paradisiacal. Mal J Anal Sci. 20(5):1181-1190.
Tun ZM, Zaw M, Aye SS, Oo SP, Naing TAA. (2018) Evaluation of plant extracts against rice blast disease caused by Pyriculariagrisea. J Agri Res. 5(1):37-43.
Doyle JJ, Doyle JL. (1897) A rapid isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull. 19:11-15.
Meghana SN, Hiremath SV. (2019) Cultural, morphological and molecular characterization of Bipolaris oryzae causing brown leaf spot of rice in northern Karnataka. J Pharm Phyto. 8(2):1235-1239.
Ratwatthananon A, Yooboon T, Bullangpoti V, Pluempanupat W. (2020) Insecticidal activity of Piper retrofractum fruit extracts and isolated compounds against Spodoptera litura. Agric Nat Res. 54:447-452.
Jamal Y, Irawati P, Fathoni A, Agusta A. (2013) Chemical constituents and antibacterial effect of essential oil of Javaneese pepper leaves (Piper retrofractum Vahl.). Media Lit. 23(2):65-72.
Panphut W. (2017) Anti-plant pathogen of bioactive metabolite from Piper retrofractum Vahl. J Eng Appl Sci. 12(23):7321-7324.
Panphut W, Budsabun T, Sangsuriya P. (2020) In vitro antimicrobial activity of Piper retrofractum fruit extracts against microbial pathogens causing infections in human and animals. Int J Micro. 1-6.
Salleh WMNHW, Hashim NA, Fabarani NP, Ahmad F. (2020) Antibacterial activity of constituents from Piper retrofractum Vahl. and Piper arborescens Roxb. Agric Conspec Sci. 85(3):269-280.
Jadid N, Arraniry BA, Hidayati D, Purwani KI, Wikanta W, Hartanti SR, Rachman RY. (2018) Proximate composition, nutritional values and phytochemical screening of Piper retrofractum Vahl. fruits. Asian Pac J Trop Biomed. 8(1):37-43.
Salleh WMNHW, Ahmad F. (2020) Phytopharmacological investigations of Piper retrofractum Vahl. – a review. Agric Conspec Sci. 85(3):193-202.
Patra B, Das MT, Dey SK. (2016) A review on Piper betle L. J Med Plant Stud. 4(6):185-192.
Ramji N, Iyer R, Chandrasekaran S. (2002) Phenolic antibacterials from Piper betle in the prevention of halitosis. J Ethno. 83:149-152.
Mula S, Banerjee D, Patro BS, Bhattacharya S, Barik A, Bandopadahyay SK, Chattopadhyay S. (2008) Inhibitory property of the Piper betle phenolics against photosensitization-induced biological damages. Bio Med Chem. 16:2932-2938.
Sharma S, Khan IA, Ali I, Ali F, Kumar M, Kumar A, Johri RK, Abdullah ST, Bani S, Pandey A, Suri KA, Gupta BD, Satti NK, Dutt P, Qazi GN. (2009) Evaluation of the antimicrobial, antioxidant and anti-inflammatory activities of hydroxychavicol for its potential use as an oral care agent. Ant Age Chem. 53:216-222.
Singburaudom N. (2015) Hydroxychavicol from Piper betle leave is an antifungal activity against plant pathogenic fungi. J Biopest. 8(2):82-92.
Suriani NL, Suprapta DN, Nazir N, Parwanayoni S, Darmadi AAK, Dewi DA, Sudatri NW, Fudholi A, Sayyed RZ, Syed A, Bahkali AH. (2020) Potential of bioactive substances of Piper caninum and Piper betle Var. Nigra leaves as biopesticide in organic Bali rice. NOT Peer-Reviewed. www.preprints.org.
Seyyedan A, Yahya F, Kamarolzaman MFF, Suhaili Z, Desa MNM, Khairi HM, Somchit MN, Fatimah CA, The LK, Salleh MZ, Zakaria ZA. (2013) Review on the ethnomedicinal, phytochemical and pharmacological properties of Piper sarmentosum: scientific justification of its traditional use. Tang Hum Med. 3(3):1-32.
Nazmul MHM, Salmah I, Syahid A, Mahmood AA. (2011) In-vitro screening of antifungal activity of plants in Malaysia. Biomed Res. 22:28-30.
Cheeptham N, Towers GHN. (2002) Light-mediated activities of some Thai medicinal plant teas. Fitoterapia. 73:651-662.
Pauli A, Kubeczka KH. (2010) Antimicrobial properties of volatile phenylpropanes. Nat Prod Comm. 5:1387-1394.
Rukachaisirikul T, Siriwattanakit P, Sukcharoenphol K, Wongvein C, Ruttanaweang P, Wongwattanavuch P, Suksamran A. (2004) Chemical constituents and bioactivity of Piper sarmentosum. J Ethno. 93:173-176.
Mueller DS, Bradley CA. (2008) Field Crop Fungicides for the North Central United States. Ames, IA and Urbana-Champaign, IL, Iowa State.
Hussain K, Hashmi FK, Latif A, Ismail Z, Sadikun A. (2012) A review of the literature and latest advances in research of Piper sarmentosum. Pharm Bio.50:1045-1052.
Lee JH, Cho S, Paik HD, Choi CW, Nam KT, Hwang SG, Kim SK. (2014) Investigation on antibacterial and antioxidant activities, phenolic and flavonoid contents of some Thai edible plants as an alternative for antibiotics. Asian-Aust. J Anim Sci.27:1461-1468.
Chanprapai P, Chavasiri W. (2017) Antimicrobial activity from Piper sarmentosum Roxb. Against rice pathogenic bacteria and fungi. J Integra Agri. 16(11):2513-2524.
Damanhori ZA, Ahmad A. (2014) A review on therapeutic potential of Piper nigrum L. (Black Pepper): the king of spices. Med Aroma Plant. 3(3):1-6.
Joshi DR, Shrestha AC, Adhikari N. (2018) A review on diversified use of the king of spices: Piper nigrum (Black Pepper). Int J Pharm Sci Res. 9(10):4089-4101.
Sukanya SL, Yamini D, Fathima SK. (2011) Eco-frindly management of Pyriculariaoryzae-the causal agent of blast of paddy. Current Bot. 2(8):46-49.
Rani SKS, Saxena N, Udaysree. (2013) Antimicrobial activity of black pepper (Piper nigrum). Global. J Pharm. 7(1):87-90.
Chouhan G, Islamuddin M, Want MY, Ozbak HA, Hemeg HA, Sahal D, Afrin F. (2015) Leishmanicidal activity of Piper nigrum bioactive fractions is interceded via apoptosis in vitro and substantiated by Th1 immuno-stimulatory potential in vivo. Front Micro. 6:1-19.
Sapam R, Kalita PP, Sarma MP, Talukdar N, Das H. (2018) Screening of phytochemicals and determination of total phenolic content, antioxidant, and antimicrobial activity of methanolic extract of Piper nigrum leaves. Indo Amer J Pharm Res. 8(2):1354-1360.
Aslam HMU, Gleason ML, Abbas A, Abdin Z, Amrao L. (2019) Molecular characterization of Magnaporthe oryzae in Pujab, Pakistan and Its in vitro suppression by fungicides and botanicals. Intl J Agric Biol.22:1459-1466.
Ahmad N, Fazal H, Abbasi BH, Farooq S. Ali M, Khan MA. (2012) Biological role of Piper nigrum L. (Black pepper): a review. Asian Pac J Trop Biomed. 1-10.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Ramkhamhaeng International Journal of Science and Technology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright Notice: a copyright on any article in the published journal is retained by the Ramkhamhaeng International Journal of Science and Technology. Readers or Users grant the right to use of the Article contained in the Content in accordance with the Creative Commons CC BY-NC-ND license and the Data contained in the Content in accordance with the Creative Commons CC BY-NC-ND.
