Determination and detection of water-extractable protein from latex gloves
Keywords:
Bradford method, Latex glove, Modified Lowry method, Natural rubber latex (NRL), Water- extractable protein (WEP)Abstract
Natural rubber latex (NRL) from the rubber tree (Hevea brasiliensis) is used in various products. However, the NRL protein is generally of concern to medical personnel and regular users. In this study, a phosphate buffer saline (PBS) extraction was introduced to facilitate the evaluation of water-extractable proteins (WEPs). The six WEPs, five different gloves, and NRL samples were determined and compared using Bradford and modified Lowry methods, respectively. An appropriate incubation time for protein extraction was at least 60 min. The standard curve of protein determination showed a good linear correlation (R2 = 0.99). Samples had higher WEP contents when determined by the modified Lowry method. In addition, WEP patterns were detected by polyacrylamide gel electrophoresis with sodium dodecyl sulphate (SDS-PAGE). The SDS-PAGE showed 7–9 bands with molecular weights ranging from 9.0 to 66.6 kDa. This indicates that PBS extraction can be efficiently used to evaluate allergies
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