The Potential of Single-Cell Oils Derived from Marine Fungus (Aspergillus pseudofelis MMERU 25) as Alternative Feedstock Sources for Biodiesel Production
Keywords:
Aspergillus pseudofelis, Biodiesel, Feedstock, Marine fungi, Single-cell oilsAbstract
The potential of single-cell oils derived from marine fungi can serve as a feedstock for biodiesel production. However, different marine fungal species exhibit distinct capabilities for high lipid production under varying conditions, resulting in variations in biodiesel characteristics. Therefore, the objective of this study is to investigate culture media conditions, the optimal duration for lipid production, and the biodiesel characteristics of Aspergillus pseudofelis MMERU 25. Cultivation was conducted using 70% MEB for 20 days and molasses (2.5, 5.0, 10.0, and 10.5 ml) mixed with 70% MEB for 15 days. Biodiesel characteristics, including chemical profiles, viscosity, acidity, methyl esters and linolenic acid content, H.H.V, and iodine value were also analyzed. The results indicate that A. pseudofelis MMERU 25 achieves the highest lipid production within 10 days in 70% MEB and reaches the maximum lipid yield at a 2.5 ml concentration of molasses in 70% MEB within 15 days. When A. pseudofelis MMERU 25 is cultured in 70% MEB for 10 days, the biodiesel quantity reaches 360 ml/kg of dry biomass. The biodiesel characteristics are consistent with previous research and the standards of biodiesel qualities by the DOEB, except for acidity, methyl esters content, and H.H.V. These parameters can be controlled to eliminate residual acids in biodiesel and adjust reaction conditions to enhance its suitability for use as a biodiesel feedstock. Therefore, biodiesel produced from marine fungi has the potential to be an alternative energy source for Thailand in the future.
References
Athenaki M, Gardeli C, Diamantopoulou P, Tchakouteu SS, Sarris D, Philippoussis A, Papanikolaou S (2018) Lipids from yeasts and fungi: physiology, production and analytical considerations. Journal of Applied Microbiology 124(2):336-367
Bardhan P, Gohain M, Daimary N, Kishor S, Chattopadhyay P, Gupta K, Mandal M (2019) Microbial lipids from cellulolytic oleaginous fungus Penicillium citrinum PKB20 as a potential feedstock for biodiesel production. Annals of Microbiology 69(11):1135-1146
Buaruang J, Manoch L, Chamswarng C, Piasai O, Yaguchi T, Kijjoa A (2015) Species of Aspergillus Section Fumigati from the Coral Reefs in the Gulf of Thailand and Andaman Sea and their Antagonistic Effects Against Plant Pathogenic Fungi. Thai Journal of Agricultural Science 48(2):87-107
Cardona HRA, Froes TQ, Souza BCD, Leite FHA, Brandão HN, Buaruang J, Kijjoa A, Alves CQ (2021) Thermal shift assays of marine-derived fungal metabolites from Aspergillus fischeri MMERU 23 against Leishmania major pteridine reductase 1 and molecular dynamics studies. Journal of Biomolecular Structure and Dynamics 40(22):11968-11976
Carvalho AK, Rivaldi JD, Barbosa JC, de Castro HF (2015) Biosynthesis, characterization and enzymatic transesterification of single cell oil of Mucor circinelloides–A sustainable pathway for biofuel production. Bioresource technology 181:47-53
Cheirsilp B, Kitcha S (2015) Solid state fermentation by cellulolytic oleaginous fungi for direct conversion of lignocellulosic biomass into lipids: fed-batch and repeated-batch fermentations. Industrial Crops and Products 66:73-80
Eamvijarn A, Manoch L, Chamswarng C, Piasai O, Visarathanonth N, Luangsa-ard JJ, Kijjoa A (2013) Aspergillus siamensis sp. nov. from soil in Thailand. Mycoscience 54(6):401-405
Freedman B, Butterfield RO, Pryde EH (1986) Transesterification kinetics of soybean oil 1. Journal of the American oil chemists’ society 63:1375-1380
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for Basidiomycetes-Application to the identification of mycorrhizae and rusts. Molecular Ecology 2:113–118
Horie Y, Abliz P, Fukushima K, Okada K, Takaki GC (2003) Two new species of Neosartorya from Amazonian soil, Brazil. Mycoscience 44(5):397-402
Kot AM, Błażejak S, Kurcz A, Gientka I, Kieliszek M (2016) Rhodotorula glutinis-potential source of lipids, carotenoids, and enzymes for use in industries. Applied Microbiology and Biotechnology 100:6103-6117
Kumar SPJ, Avanthi A, Chintagunta AD, Gupta A, Banerjee R (2020) Oleaginous lipid: a drive to synthesize and utilize as biodiesel. Springer, New Delhi, pp 105-129
Lamers D, van Biezen N, Martens D, Peters L, van de Zilver E, Jacobs-van Dreumel N, Wijffels RH, Lokman C (2016) Selection of oleaginous yeasts for fatty acid production. BMC Biotechnology 16(1):1-10
Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Applied Microbiology and Biotechnology 80:749-756
Longmatcha K, Chaiyasaeng W, Yeemin T, Sutthacheep M, Buaruang J (2022) Screening and optimization of oleaginous marine fungi for lipid production as a possible source for biodiesel. Ramkhamhaeng International Journal of Science and Technology 5(2):8-17
Manoch L, Piasai O, Dethoup T, Kokaew J, Eamvijarn A, Piriyaprin S (2009) Morphological studies of slime molds, sordariaceous fungi, and an endophytic synnemata fungus. The Journal of The Microscopy Society of Thailand 23:25-29
Martins F, Felgueiras C, Smitkova M, Caetano N (2019) Analysis of fossil fuel energy consumption and environmental impacts in European countries. Energies 12(6):964
Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renewable Energy 34(1):1-5
Mhlongo SI, Ezeokoli OT, Roopnarain A, Ndaba B, Sekoai PT, Habimana O, Pohl CH (2021) The potential of single-cell oils derived from filamentous fungi as alternative feedstock sources for biodiesel production. Frontiers in Microbiology 12:57
Narala RR, Garg S, Sharma KK, Thomas-Hall SR, Deme M, Li Y, Schenk PM (2016) Comparison of microalgae cultivation in photobioreactor, open raceway pond, and a two-stage hybrid system. Frontiers in Energy Research 4:29
Rayner, RW (1970) A Mycological Colour Chart. Commonwealth Mycological Institute, Kew, Surrey
Reis CER, Carvalho AKF, Bento HB, de Castro HF (2019) Integration of microbial biodiesel and bioethanol industries through utilization of vinasse as substrate for oleaginous fungi. Bioresource Technology Reports 6:46-53
Reis CER, Valle GF, Bento HB, Carvalho AK, Alves TM, de Castro HF (2020) Sugarcane by-products within the biodiesel production chain: Vinasse and molasses as feedstock for oleaginous fungi and conversion to ethyl esters. Fuel 277:118064
Sargeant LA, Jenkins RW, Chuck CJ (2017) Lipid‐based biofuels from oleaginous microbes. Biofuels and Bioenergy 227-240
Strobel GA (2015) Bioprospecting-fues from fungi. Biotechnol Lett 37:973-982
Singh D, Sharma D, Soni SL, Sharma S, Sharma PK, Jhalani A (2020) A review on feedstocks, production processes, and yield for different generations of biodiesel. Fuel 262:116553
Talebian-Kiakalaieh A, Amin NAS, Mazaheri H (2013) A review on novel processes of biodiesel production from waste cooking oil. Applied Energy 104:683-710
Vicente G, Bautista LF, Rodríguez R, Gutiérrez FJ, Sádaba I, Ruiz-Vázquez RM, Torres Martínez S, Garre V (2009) Biodiesel production from biomass of an oleaginous fungus. Biochemical Engineering Journal 48(1):22-27
Vicente G, Martınez M, Aracil J (2004) Integrated biodiesel production: a comparison of different homogeneous catalysts systems. Bioresource technology 92(3):297-305
Wethered JM, Metcalf EC, Jennings DH (1985) Carbohydrate metabolism in the fungus Dendryphiella salina: VIII. The contribution of polyols and ions to the mycelial solute potential in relation to the external osmoticum. New phytologist 101(4):631-649
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18(1):315-322
Zabeti N, Bonin P, Volkman JK, Guasco S, Rontani JF (2010) Fatty acid composition of bacterial strains associated with living cells of the haptophyte Emiliania huxleyi. Organic Geochemistry 41(7):627-636
Zhang X, Yan S, Tyagi RD, Drogui P, Surampalli RY (2014) Ultrasonication assisted lipid extraction from oleaginous microorganisms. Bioresource technology 158:253-261
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Ramkhamhaeng International Journal of Science and Technology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright Notice: a copyright on any article in the published journal is retained by the Ramkhamhaeng International Journal of Science and Technology. Readers or Users grant the right to use of the Article contained in the Content in accordance with the Creative Commons CC BY-NC-ND license and the Data contained in the Content in accordance with the Creative Commons CC BY-NC-ND.
