Comparative Study on Biological Activities of Steamed and Non - Steamed Ginger Extracts

  • Naphatsaran Roekruangrit Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Nuanjan Jaiarree Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Arunporn Itharat Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Preecha Wanichsetakul Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Sumalee Panthong Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Sunita Makchuchit Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
  • Saovapak Poomirat Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
Keywords: Ginger, NO, PGE2, Cytotoxic, Steamed, Non - steamed

Abstract

Ginger is used for treating motion sickness, nausea and vomiting. The traditional Thai method of preparation is to steam the rhizome before preparing the remedy. There is no report comparing the biological activity and quality of extracts from steamed and non-steamed rhizomes. The objective was to compare the anti-inflammatory activities, cytotoxicity and quality of steamed and non-steamed ginger extracts. All extracts were tested for their potential anti-inflammatory properties via the inhibitory effect on NO and PGE2 production, and cytotoxic activity via sulforhodamine B assay. Loss on drying, total ash and acid - insoluble ash determinations were used as quantitation evaluation to standardize the ginger extracts. The results showed that the quality of all samples was within standard guidelines. The anti-inflammatory NO inhibition effect was higher in the steamed ginger ethanol extract (AZOE) than in the non-steamed ginger ethanol extract (HZOE), but lower than prednisolone as a positive control, with IC50 values of 13.47 ± 0.20, 19.64 ± 0.33 and 1.33 ± 0.02 µg/ml, respectively. The effect of PGE2 inhibition of AZOE, HZOE and positive control, had IC50 values of 0.40 ± 0.06, 0.63 ± 0.02 and 0.066 ± 0.004 µg/ml, respectively. However, the steamed and non-steamed ginger ethanol extracts were not cytotoxic to either SKOV - 3 or HeLa cells. Steamed ginger had a higher inhibition effect on NO and PGE2 release than non - steamed ginger. These results support the traditional Thai method of steaming the rhizome before preparing the medicine.

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Published
2019-10-31
How to Cite
Roekruangrit, N., Jaiarree, N., Itharat, A., Wanichsetakul, P., Panthong, S., Makchuchit, S., & Poomirat, S. (2019). Comparative Study on Biological Activities of Steamed and Non - Steamed Ginger Extracts. Science & Technology Asia, 24(4), 94-101. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/183141
Section
Biological sciences