Cytogenetic Studies of Wrinkle-lipped Bat, Chaerephon plicatus, from Tham Pra Thun Non-hunting Area, Uthai Thani Province, Thailand

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เผยแพร่แล้ว: ก.ย. 30, 2026

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Kanya Anukulthanakorn
Biology and Biotechnology Program, Department of Science, Faculty of Science and Technology, Nakhon Sawan Rajabhat University, Nakhon Sawan, Muang 60000, Thailand
Daramanee Khummok
Biology and Biotechnology Program, Department of Science, Faculty of Science and Technology, Nakhon Sawan Rajabhat University, Nakhon Sawan, Muang 60000, Thailand
Krittikan Aiamsanthia
Biology and Biotechnology Program, Department of Science, Faculty of Science and Technology, Nakhon Sawan Rajabhat University, Nakhon Sawan, Muang 60000, Thailand
Weerachet Puppako
Protected Area Regional Office 12, Nakhon Sawan, Muang 60000, Thailand
Alongklod Tanomtong
Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Muang 40002, Thailand

บทคัดย่อ

Chromosome and morphometric analyses of the wrinkle-lipped bat, Chaerephon plicatus (Buchannan, 1800), from Khao Khong Chai cave, Tham Pra Thun Non-hunting area in Uthai Thani province, Thailand were studied. Bat chromosomes in both males and females were determined by the squash technique using bone marrow and testis following conventional staining and Ag-NOR banding technique. The results showed that the diploid chromosome number of C. plicatus was 2n = 48, and the fundamental number (NF) was 56 in both male and female. The types of autosomes were 4 large metacentric, 2 medium metacentric, 8 medium telocentric, 4 small metacentric, and 28 small telocentric chromosomes. The X chromosome was medium metacentric, and the Y chromosome was small telocentric. The NOR chromosome markers were found on chromosome pair 6 telocentric and 9 metacentric. The karyotype of C. plicatus was as follows:


                                    2n (48) = Lm4 + Mm2 + Mt8 + Sm4 + St28 + sex chromosomes


These findings provide additional cytogenetic information for C. plicatus and contribute to the understanding of chromosomal variation within the family Molossidae.

Article Details

รูปแบบการอ้างอิง
Anukulthanakorn, K., Daramanee Khummok, Krittikan Aiamsanthia, Weerachet Puppako, & Alongklod Tanomtong. (2026). Cytogenetic Studies of Wrinkle-lipped Bat, Chaerephon plicatus, from Tham Pra Thun Non-hunting Area, Uthai Thani Province, Thailand. Science & Technology Asia, 31(3), 335–344. สืบค้น จาก https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/262385
ฉบับ
Vol.31 No.3 (July-September 2026)
ประเภทบทความ
Biological sciences
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อนุญาตภายใต้เงื่อนไข Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

เอกสารอ้างอิง

Simmons NB. Order Chiroptera. In: Wilson DE, Reeder DM, editors. Mammal species of the world: a taxonomic and geographic reference (3rd ed.). Baltimore: The Johns Hopkins University Press; 2005. p. 312–529.

Lekagul B, McNeely JA. Mammals of Thailand (2nd ed.). Bangkok: Saha Karn Bhaet; 1988.

Buchannan F. XXVII. Description of the Vespertilio plicatus. Trans Linn Soc Lond. 1800;5(1):261-263.

Thong VD. Taxonomic and distribution assessment of Chaerephon plicatus (Chiroptera: Molossidae) from Vietnam. Tap Chi Sinh Hoc. 2014;36(4):479-486.

Freeman PW. A multivariate study of the family Molossidae (Mammalia: Chiroptera): Morphology, Ecology, Evolution. Fieldiana Zoo New Ser. 1981;(7):1–173.

Howell WM, Black DA. Controlled silverstaining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980;36:1014-1015.

Verma RS, Babu A. Human chromosomes: principles and techniques (2nd ed.). New York: McGraw-Hill; 1995.

Turpin R, Lejeune J. Les chromosomes humains. Paris: Gauthier-Villars; 1965.

Harada M, Minezawa M, Takada S, Yenbutra S, Nunpakdee P, Ohtani S. Karyological analysis of 12 species of bats from Thailand. Caryologia. 1982;35(2):269-278.

Sreepada KS, Koubinova D, Konecny A, Koubek P, Rab P, Rabova M, et al. Karyotypes of three species of molossis bats (Molossidae, Chiroptera) from India and western Africa. Folia Zool. 2008;57(4):347-357.

Levan A, Fredga A, Sandberg AA. Nomenclature of centromeric position on chromosome. Hereditas. 1964;52:201-220.

Velleth M, Heller KG, Pfeiffer RA, Hameister H. A comparative ZOO-FISH analysis in bats elucidates the phylogenetic relationships between Megachiroptera and five microchiropteran families. Chromosome Res. 2002;10:477-497.

Gold JR, Ellison JR. Silver staining for nucleolar organizing regions of vertebrate chromosomes. Stain Technol. 1983;58(1):51-55.

Gold JR, Li YC, Shiply NS, Powers PK. Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J Fish Biol. 1990;37(4):563-575.

Suzuki H, Sakurai S, Mutsuda Y. Rat 5S rRNA spacer sequence and chromosomal assignment of the gene to the extreme terminal region of chromosome 19. Cytogenet Cell Genet. 1996;72(1):1-4.

Supanuam P, Tanomtong A, Khunsook S, Pintong K, Sanoamuang L. Karyological analysis and morphometric of the lesser asiatic house bat, Scotophilus kuhlii (Chiroptera, Vespertilionidae). Cytologia. 2012;77(3):401-411.

Jantarat S, Supanuam P, Tanontong A, Khunsok S, Prakrongrak N, Kaewsri S. Chromosome analysis and morphometric of intermediate roundleaf bat, Hipposideros larvatus (Chiroptera, Hipposideridae) by conventaional, GTG-banding and Ag-NOR banding techniques. Cytologia. 2014;79(4):445-456.

Patawang I, Tengjaroenkul B, Supanuam P, Kakampuy W, Khunsook S, Tanomtong A. Chromosome analysis and morphometric of croslet horseshoe bat, Rhinolophus coelophyllus and least horseshoe bat, Rhinolophus pusillus (Chriroptera, Rhinolophidae). Cytologia.

;82(5):471-480.